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Week12
From 2009.igem.org
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| - | 1.FACS with Host as Control | + | 1.FACS with Host as Control . |
| - | 2.Transformations done with new host strain | + | 2.We got one new host strain which is lacI deleted at the same time both kanamycin and ampicillin sensitive. |
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| + | 3.Competent cells were prepared from this strain. | ||
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| + | 4.Transformations done with new host strain E.coli MG1655- some problem with ampicillin plates. There was some | ||
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| + | colonies on the control plate also. Problem thought was that there may be some contamination while preparing | ||
| + | |||
| + | competent cells for the new hosts. | ||
Latest revision as of 01:45, 22 October 2009
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1.FACS with Host as Control . 2.We got one new host strain which is lacI deleted at the same time both kanamycin and ampicillin sensitive. 3.Competent cells were prepared from this strain. 4.Transformations done with new host strain E.coli MG1655- some problem with ampicillin plates. There was some colonies on the control plate also. Problem thought was that there may be some contamination while preparing competent cells for the new hosts. |
