Team:Heidelberg/Project dual assay plasmid

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Revision as of 22:17, 21 October 2009

Dual Assay Plasmid

Introduction

which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive and the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection efficiencies. Unfortunately the repeated cloning of the construct, which is shown in Fig. 1, was not successful.

Figure 1: The planned dual assay plasmid contained a site for promoter exchange, where one can put the promoter, that has to be tested. This promoter is upstream of a GFP gene and regulates its transcription. This gene is followed by a Jet promoter that constitutively promotes transcription of a mCherry, serving as a reference.

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 === Introduction ===
-which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection efficiencies. Unfortunately the repeated cloning of the construct, which is shown in Fig 1, was not successful.
+which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive and the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection efficiencies. Unfortunately the repeated cloning of the construct, which is shown in Fig. 1, was not successful.
-[[Image:Dual_plasmid.png|thumb|left|300px|<div style="text-align:justify;">'''Fig. 1: The planned dual assay plasmid contained a site for promoter exchange, where one can put the promoter, that has to be tested. This promoter is upstream of a GFP gene and regulates its transcription. This gene is followed by a Jet promoter that constitiutively promotes transcription of a mCherry, serving as a reference.</div>]]
+[[Image:Dual_plasmid.png|thumb|left|300px|<div style="text-align:justify;">'''Figure 1: The planned dual assay plasmid contained a site for promoter exchange, where one can put the promoter, that has to be tested. This promoter is upstream of a GFP gene and regulates its transcription. This gene is followed by a Jet promoter that constitutively promotes transcription of a mCherry, serving as a reference.</div>]]
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