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Revision as of 20:55, 9 July 2009 (view source) Lor18 (Talk | contribs) (→DNA precipitation with sodium acetate) ← Older edit		Revision as of 21:17, 20 July 2009 (view source) Lor18 (Talk | contribs) (→DNA precipitation with sodium acetate) Newer edit →
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	*'''Ethanol 70%'''		*'''Ethanol 70%'''
	*'''ddH2O'''		*'''ddH2O'''
-	*'''Previously cut plasmid'''	+	*'''DNA sample'''
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Revision as of 21:17, 20 July 2009

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

DNA precipitation with sodium acetate

(estimated time: 1 hour)

Add 1/10 DNA solution volume of sodium acetate 3 M Add 2.5 DNA solution volume of absolute ethanol Freeze at -80°C for 30 min Centrifuge at 13000 rpm, 4°C for 20 min Decant supernatant Add 50 µl of 70% ethanol Centrifuge at 13000 rpm, 4°C for 20 min Remove all supernatant with a pipette Air dry pellet until ethanol is totally removed Elute with 5-10 µl of ddH2O

Sodium acetate Absolute ethanol Ethanol 70% ddH2O DNA sample

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