Team:TUDelft/Literature

From 2009.igem.org

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(New page: {{Template:TUDelftiGEM2009}} ''Page is under construction'' <br> ='''Project Overview'''= __TOC__ =='''Our abstract'''== <br> Finally TUDelft team has a project!!! ===Self-destructive plas...)
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''Page is under construction''
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='''Literature'''=
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<br>
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='''Project Overview'''=
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__TOC__
__TOC__
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=='''Our abstract'''==
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==Part 3: Conjugation==
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<br>
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##Thorsted, P. B., D. P. Macartney, P. Akhtar, A. S. Haines, N. Ali, P. Davidson, T. Stafford, M. J. Pocklington, W. Pansegrau, B. M. Wilkins, E. Lanka, and C. M. Thomas. 1998. [http://www.ncbi.nlm.nih.gov/nuccore/113911681?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum Complete sequence of the IncPbeta  plasmid R751: implications for evolution and organisation of the IncP backbone]. J. Mol. Biol. 282:969-990.
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Finally TUDelft team has a project!!!
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===Self-destructive plasmid===
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[[Image:TUDelft_logo.png|50px|right|frame]]
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'''Research question:'''
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Can we achieve cell-to-cell communication:
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#Using delay device
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#Using self-destructive plasmid
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#Between two F+ cells
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'''Goals:'''
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<br>
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*Build a delay device that can change the timing of transcribing the target protein
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*Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
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*Construct a modified F plasmid that will allow F+/F+ conjugation
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<br>
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'''Why?'''
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*To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
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*To generate a pulse in the expression of a certain gene
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*To control the amplitude of the pulse created by the plasmid
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*Because it is cool!!!
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<br><br>
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'''How?'''
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Not known yet :D
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<br><br>
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'''Applications:'''
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*Cell synchronization
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*Selectable marker recycling
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*Metabolic control analysis
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*Recombination
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*…
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<br>
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==Project Details==
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##Pansegrau, W., E. Lanka, P. T. Barth, D. H. Figurski, D. G. Guiney, D. Haas, D. R. Helinski, H. Schwab, V. A. Stanisich, and C. M. Thomas. 1995. [http://www.ncbi.nlm.nih.gov/nuccore/508311?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum Complete nucleotide sequence of Birmingham IncP alpha plasmids]. Compilation and comparative analysis. J. Mol. Biol. 239:623-663.
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<br>
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==The Experiments==
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##Frost, L., K. Ippen-Ihler, and M. Skurray. 1995. [http://www.ncbi.nlm.nih.gov/nuccore/9507713?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum Analysis of the sequence and gene products of the transfer region of the F sex factor]. Microbiol. Rev. 58:162-210.
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<br>
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==Results==
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##Plasmid Biology. Barbara E. Funnell and Gregory J. Phillips, eds. American Society for Microbiology Press, Washington, DC, 2004. Chapter 9.
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<br>
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##Garcillán-Barcia MP, de la Cruz F. Why is entry exclusion an essential feature of conjugative plasmids? Plasmid. 2008;60:1–18. doi: 10.1016/j.plasmid.2008.03.002.
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##Ou, J. T. 1980. Role of surface exclusion genes in lethal zygosis inEscherichia coli K12 mating. Molecular and General Genetics178:573–581.
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##Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720–6729 (1996).
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##Haase J, Lurz R, Grahn A M, Bamford D H, Lanka E. Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex. J Bacteriol. 1995;177:4779–4791.
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##Li, P. L., I. Hwang, H. Miyagi, H. True, and S. K. Farrand. 1999. Essential components of the Ti plasmid trb system, a type IV macromolecular transporter. J. Bacteriol. 181:5033-5041.

Revision as of 12:10, 12 July 2009

Literature

Contents

Part 3: Conjugation

    1. Thorsted, P. B., D. P. Macartney, P. Akhtar, A. S. Haines, N. Ali, P. Davidson, T. Stafford, M. J. Pocklington, W. Pansegrau, B. M. Wilkins, E. Lanka, and C. M. Thomas. 1998. [http://www.ncbi.nlm.nih.gov/nuccore/113911681?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum Complete sequence of the IncPbeta plasmid R751: implications for evolution and organisation of the IncP backbone]. J. Mol. Biol. 282:969-990.
    1. Pansegrau, W., E. Lanka, P. T. Barth, D. H. Figurski, D. G. Guiney, D. Haas, D. R. Helinski, H. Schwab, V. A. Stanisich, and C. M. Thomas. 1995. [http://www.ncbi.nlm.nih.gov/nuccore/508311?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum Complete nucleotide sequence of Birmingham IncP alpha plasmids]. Compilation and comparative analysis. J. Mol. Biol. 239:623-663.
    1. Frost, L., K. Ippen-Ihler, and M. Skurray. 1995. [http://www.ncbi.nlm.nih.gov/nuccore/9507713?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum Analysis of the sequence and gene products of the transfer region of the F sex factor]. Microbiol. Rev. 58:162-210.
    1. Plasmid Biology. Barbara E. Funnell and Gregory J. Phillips, eds. American Society for Microbiology Press, Washington, DC, 2004. Chapter 9.
    1. Garcillán-Barcia MP, de la Cruz F. Why is entry exclusion an essential feature of conjugative plasmids? Plasmid. 2008;60:1–18. doi: 10.1016/j.plasmid.2008.03.002.
    1. Ou, J. T. 1980. Role of surface exclusion genes in lethal zygosis inEscherichia coli K12 mating. Molecular and General Genetics178:573–581.
    1. Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720–6729 (1996).
    1. Haase J, Lurz R, Grahn A M, Bamford D H, Lanka E. Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex. J Bacteriol. 1995;177:4779–4791.
    1. Li, P. L., I. Hwang, H. Miyagi, H. True, and S. K. Farrand. 1999. Essential components of the Ti plasmid trb system, a type IV macromolecular transporter. J. Bacteriol. 181:5033-5041.
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