Virginia Commonwealth/14 July 2009
From 2009.igem.org
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[[User:Trentay|Trentay]] 15:13, 14 July 2009 (UTC) | [[User:Trentay|Trentay]] 15:13, 14 July 2009 (UTC) | ||
''Kevin and Adam'' | ''Kevin and Adam'' | ||
- | *construct biobrick device pSB4C5, | + | *construct biobrick device (pSB4C5, BBa_E0240, BBa_J23100), and (pSB4C5, BBa_E0240, BBa_J23106) |
[[User:Bussingkm|Bussingkm]] 15:20, 14 July 2009 (UTC) | [[User:Bussingkm|Bussingkm]] 15:20, 14 July 2009 (UTC) | ||
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Revision as of 15:28, 14 July 2009
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Contents |
Tuesday 14 July 2009
Results
Maria and Afton
- (+) Control: NEB 10 beta on LB
- lawn growth
- (+) Control: NEB 10 beta on LB shocked
- lawn growth
- (-) Control: NEB 10 beta on Cm
- no growth
- NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
- Approximately 30 small colonies
Trentay 13:44, 14 July 2009 (UTC) Kevin and Adam
- Miniprep results:
Bussingkm 15:21, 14 July 2009 (UTC)
Tasks
Maria and Afton
- Digest promoters: 101, 102, 103, 104
- Possible Ligation of promoters with parts J06702, pSB4C5
Trentay 15:13, 14 July 2009 (UTC) Kevin and Adam
- construct biobrick device (pSB4C5, BBa_E0240, BBa_J23100), and (pSB4C5, BBa_E0240, BBa_J23106)
Bussingkm 15:20, 14 July 2009 (UTC)
Wetlab
Maria and Afton
- Spectophotometry of promoters: 101, 102, 103, 104
- Digestion of promoters
- Electrophoresis of promoters
Trentay 15:13, 14 July 2009 (UTC) Kevin and Adam
- pSB4C5, BBa_E0240, and BBa_J06702 were frozen in box C-11 of the glycerol stocks
Bussingkm 15:22, 14 July 2009 (UTC)