Team:Groningen/Brainstorm/Rainbow Bacteria

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Revision as of 11:54, 3 May 2009

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Tianjin University 2008 (China) – Made use of the CRE/LoxP combination (together with other strategies) to combine DNA and make “Foolproof Plasmid Self-Assembly Systems”.

MIT 2006 (USA) – Mention of mutant LoxP sites to prevent inversion of sequences (Lox66 and Lox71)

Imperial College 2006 (London) – Biological oscillator by using the CRE/Lox system as a switch by incorporating DNA to prevent downstream transcription. In the end used Quorum Sensing to create a predator – pray system.

Paris 2007 (France) – (Synthetic Multicellular Bacterium) Use of CRE/Lox system to influence differentiation in germ line, because it was readily available, largely used and well described. Low concentrations of DAP triggered the transcription of Cre-recombinase (under the influence of DAP-sensitive promoter), which caused the removal of a gene required for bacterial reproduction by site-specific recombination (SSR), and resulted in the activation of a gene for the production of DAP by them. The SSR sites might become a burden.

Berkeley 2007 (England) – Registration of recombinase parts (Cre), Bacteriophage P1-derived Cre-Lox.

USTC 2008 (China) – made use of Cre to cut out “sender” genes upon receiving a signal from a sender cell and thereby creating a receiver cell.

Tsinghua 2008 – use of the Cre/Lox system in their second project, but is unclear why they used it.
Caltech 2008 - Wilfred Not Cre/LoxP but it does induce variation in the population
Hong kong 2008 Wilfred Look at the overlapping T7 promoters for variation in response

Utah State University – use of GFP as a reporter gene to indicate the maximum production level of PHB.

University of Sheffield – use of GFP as a reporter gene upon detection of a pathogen by a receiver protein.

UNIPV-Pavia – use of GFP and RFP as reporter genes in electronically based circuit (on/off).

TUDelft – use of different method to produce colors, but results in this area were not very promising (also due to ordered parts from iGEM).

Tokyo Tech – use of GFP as a reporter gene in their “coli touch” display experiment.

Paris – use of (E)CFP, YFP, GFP and mRFP in the construction of a oscillating time clock project.

Newcastle University – use of a XFP as a reporter gene to indicate the amount of increase in POP’s.

Fluorescent protein sequences

Workable are the wild-type GFP (and a few mutants), RFP (and cherry version)
Not-workable (no info) are YFP and CFP (yellow and cyan), OFP (orange), SBFP2 (blue) and a few mutant proteins.

Recombinase

Workable are Cre DNA recombinase, and Hin invertase
No info (not-workable) are mutant (altered stop/start codon) Cre versions, and a few other enzymes like integrase.

Recombination sites

Membrane proteins

Only a few mentioned in the lists of receptors, transporters, channels, pumps and “other proteins”, maybe 40 in total, but I am not familiar with these names ([http://en.wikipedia.org/wiki/Toll-like_receptor TLR], Omp).

All of the workable protein sequences are listed as “1 star” in the availability list!!

J. Livet et al., Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system, 2007, Nature, Vol. 450, 56-63.
J. W. Lichtman, J. Livet & J. R. Sanes, A technicolour approach to the connectome, 2008, Nature Reviews neuroscience, Vol. 9, 417-422.

@@ Line 26: / Line 26: @@
 :*[https://2008.igem.org/Team:Tsinghua Tsinghua] 2008 – use of the Cre/Lox system in their second project, but is unclear why they used it.
 :*[https://2008.igem.org/Team:Caltech/Project/Population_Variation Caltech 2008] - [[User:Wilfred| Wilfred]] <i>Not Cre/LoxP but it does induce variation in the population</i>
+:*[https://2008.igem.org/Team:HKUSTers/Project Hong kong 2008] [[User:Wilfred| Wilfred]] <i>Look at the overlapping T7 promoters for variation in response</i>
 ==Use or mention of XFP’s==