Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

From 2009.igem.org

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=='''Plasmid extraction'''==
=='''Plasmid extraction'''==
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''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
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''(Estimated time: 1 hour)''
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Revision as of 21:16, 20 July 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 1 hour)

  • Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]










  • 5 ml or 9 ml (for higher yields) overnight mini-culture
  • QIAGEN QIAprep Spin Miniprep Kit




Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction"