Team:KULeuven/22 July 2009

From 2009.igem.org

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(Wet Lab)
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== Project ==
== Project ==
The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems:
The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems:

Revision as of 10:26, 23 July 2009

Project progress

Wet Lab

  • Grown parts in liquid medium

Project

The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems: Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.

There are two solutions to this problem:

  1. Construct a different vanillin receptor
    • Using VirA/VirG, a phenol receptor in agrobacterium .
  2. Detect an alternative substance that has a linear relationship with the vanillin concentration
    • Vanillin inhibits the Ahl receptor
    • Synthesis of another signaling molecule that will be sensed