Team:KULeuven/Notebook/Blue Light Receptor

From 2009.igem.org

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==24 July==
==24 July==
* PCR on primers and tested on agarose gel
* PCR on primers and tested on agarose gel
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= Week 1: 6 July 2009 - 12 July 2009 =
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<!-- {{Team:KULeuven/EditableTemplateH2|Monday|Team:KULeuven/6_July_2009/BlueLightReceptor}} -->
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<!-- {{Team:KULeuven/EditableTemplateH2|Monday|Team:KULeuven/12_July_2009/BlueLightReceptor}} -->
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= Week 2: 13 July 2009 - 19 July 2009 =
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<!-- {{Team:KULeuven/EditableTemplateH2|Monday|Team:KULeuven/13_July_2009/BlueLightReceptor}} -->
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= Week 3: 20 July 2009 - 26 July 2009 =
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= Week 4: 27 July 2009 - 2 August 2009 =
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<!-- {{Team:KULeuven/EditableTemplateH2|Monday|Team:KULeuven/2_August_2009/BlueLightReceptor}} -->

Revision as of 13:30, 27 July 2009


13 July

  • YCGF Gene, natural available in [http://ecoliwiki.net/colipedia/index.php/ycgF:Gene 3 E. Coli strains]. Can use the promotor of Ycgf gene for expression
  • Probable strain K12
  • Promotor voor YCGF gene:
    • AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG (bron: [www.ncbi.nlm.nih.gov])

14 July

  • Primer selection for the promotor [Done]
    • Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]

15 July

  • Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]

23 July

  • Primers for the blue light receptor have arrived

24 July

  • PCR on primers and tested on agarose gel

Week 1: 6 July 2009 - 12 July 2009

Week 2: 13 July 2009 - 19 July 2009

Week 3: 20 July 2009 - 26 July 2009

Week 4: 27 July 2009 - 2 August 2009

Retrieved from "http://2009.igem.org/Team:KULeuven/Notebook/Blue_Light_Receptor"