Team:British Columbia/Project

From 2009.igem.org

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(The Experiments)
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=== The Experiments ===
=== The Experiments ===
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1. Random mutagenesis of pBAD promoter sequence.
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# Random mutagenesis of pBAD promoter sequence.
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2. Quantification of mutant promoter-driven GFP fluorescence.
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# Quantification of mutant promoter-driven GFP fluorescence.
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3. Sequencing of select mutants.
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# Sequencing of select mutants.
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4. BioBrick design and submission.
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# BioBrick design and submission.
=== Results ===
=== Results ===

Revision as of 06:12, 2 June 2009

IGEMFinalGoldDNA-logo.jpg Home The Team The Project Parts Submitted to the Registry Modeling Sponsor Us! Biosensor Sensitivity Notebook Biosensor Logic Gate Notebook Bibliography


Contents

Overall project

1. Sensitivity of a biosensor

2. Logic gates

Biosensor

Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.

The Experiments

  1. Random mutagenesis of pBAD promoter sequence.
  2. Quantification of mutant promoter-driven GFP fluorescence.
  3. Sequencing of select mutants.
  4. BioBrick design and submission.

Results

Logic Gates

Miscellaneous Data

Biobricks.zip - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here]

Links

http://mobyle.pasteur.fr/cgi-bin/portal.py - a set of web-accessible bioinformatics tools including Mfold, which determines 2D RNA structure and draws it

http://frodo.wi.mit.edu/ - Primer3, a primer design program