Team:Calgary/21 May 2009

(Difference between revisions)

Revision as of 17:32, 28 July 2009

University of Calgary

NOTEBOOK PAGE INDEX Carol Chinmoyee Emily Fahd Iman Jamie Jeremy Katie Kevin Mandy Patrick Prima Stefan Vicki		JULY 23, 2009 (DATE)

		CAROL Verification of amplification of luxCDABE from polymerase chain reaction (PCR) Prepared 0.7% Agarose gel Used orange G dye Ran gel at 90V for 90 minutes RESULTS: From the results, there is contamination in the negative control, but it did amplify the 6KB LUXCDABE. The large bands is a result of loading too much DNA.

		CHINMOYEE Descriptive Title of What You're Doing WIKI CODING HERE

		EMILY Descriptive Title of What You're Doing WIKI CODING HERE

		FAHD Descriptive Title of What You're Doing WIKI CODING HERE

		IMAN Descriptive Title of What You're Doing WIKI CODING HERE

		JAMIE Descriptive Title of What You're Doing WIKI CODING HERE

		JEREMY Descriptive Title of What You're Doing WIKI CODING HERE

		KATIE Descriptive Title of What You're Doing WIKI CODING HERE

		KEVIN Descriptive of What You're Doing WIKI CODING HERE

		MANDY Descriptive Title of What You're Doing WIKI CODING HERE

		PATRICK Descriptive Title of What You're Doing WIKI CODING HERE

		PRIMA Descriptive Title of What You're Doing WIKI CODING HERE

		STEFAN Descriptive Title of What You're Doing WIKI CODING HERE

		VICKI Descriptive Title of What You're Doing WIKI CODING HERE

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-VERIFICATION OF AMPLIFICATION OF <i>LUXCDABE</i> FROM POLYMERASE CHAIN REACTION (PCR)
+Verification of amplification of <i>luxCDABE</i> from polymerase chain reaction (PCR)
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-* PREPARED 0.7% AGARROSE GEL
+* Prepared 0.7% Agarose gel
-* USED ORANGE G DYE
+* Used orange G dye
-* RAN GEL AT 90V FOR 90 MINUTES
+* Ran gel at 90V for 90 minutes
 RESULTS:
 [[Image:luxCDABEPCR.png]]
-*FROM THE RESULTS, THERE IS CONTAMINATION IN THE NEGATIVE CONTROL, BUT IT DID AMPLIFY THE 6KB <i>LUXCDABE</i>. THE LARGE BANDS IS A RESULT OF LOADING TOO MUCH DNA
+*From the results, there is contamination in the negative control, but it did amplify the 6KB <i>LUXCDABE</i>. The large bands is a result of loading too much DNA.
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