Team:Calgary/28 May 2009

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Revision as of 18:51, 28 July 2009

University of Calgary

NOTEBOOK PAGE INDEX Carol Chinmoyee Emily Fahd Iman Jamie Jeremy Katie Kevin Mandy Patrick Prima Stefan Vicki		MAY 28, 2009

		CAROL Descriptive Title of What You're Doing WIKI CODING HERE: bullet bold italics underline Named link: [http://en.wikipedia.org Wikipedia] link to books using ISBNs will end up like:

		CHINMOYEE Descriptive Title of What You're Doing WIKI CODING HERE

		EMILY Sequencing of LuxOD47E in psB1AC3 Objective: To veriffy the presence of the LuxOD47E gene in the psB1AC3 BBK vector Size of plasmid Backbone: 3055 bp Size of the gene of interest: 1362 bp Total size: 4417 bp Sequencing needs 100ng of DNA/ 1 kb 4.4kb x 100ng/kb = 440ng Concentration of C2= 92.9ng/uL 440ng/ 92.9 ng/uL = 4.8 uL DNA Diluted 4.8 uL DNA (LuxOD47E BBK C2) with 5.2 uL ddH20 and sent down to Univeristy of Calgary Sequencing Labs with VF2 and VF1 primers. Results: Sequencing came back and results indicated that the gene if interest, LuxOD47E was not in the psB1AC3 vector. We will have to go back to the gene in the pCR2.1-TOPO vector and try to get it into the psB1AC3 vector.

		FAHD Descriptive Title of What You're Doing WIKI CODING HERE

		IMAN Descriptive Title of What You're Doing WIKI CODING HERE

		JAMIE Descriptive Title of What You're Doing WIKI CODING HERE

		JEREMY Descriptive Title of What You're Doing WIKI CODING HERE

		KATIE Descriptive Title of What You're Doing WIKI CODING HERE

		KEVIN Descriptive of What You're Doing WIKI CODING HERE

		MANDY Descriptive Title of What You're Doing WIKI CODING HERE

		PATRICK Descriptive Title of What You're Doing WIKI CODING HERE

		PRIMA Descriptive Title of What You're Doing WIKI CODING HERE

		STEFAN Descriptive Title of What You're Doing WIKI CODING HERE

		VICKI Descriptive Title of What You're Doing WIKI CODING HERE

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-JULY 23, 2009 (DATE)
+MAY 28, 2009
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-Descriptive Title of What You're Doing
+Sequencing of LuxOD47E in psB1AC3
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-WIKI CODING HERE
+Objective: To veriffy the presence of the LuxOD47E gene in the psB1AC3 BBK vector
+<BR>
+Size of plasmid Backbone:  3055 bp
+<Br>
+Size of the gene of interest: 1362 bp
+<Br>
+Total size:                            4417 bp
+<Br>
+Sequencing needs 100ng of DNA/ 1 kb
+<Br>
+.4kb x 100ng/kb = 440ng
+	<Br>
+Concentration of C2= 92.9ng/uL
+<Br>
+ng/ 92.9 ng/uL = 4.8 uL DNA
+<Br>
+Diluted 4.8 uL DNA (LuxOD47E BBK C2) with 5.2 uL ddH20 and sent  down to Univeristy of Calgary Sequencing Labs with VF2 and VF1 primers.
+<Br>
+Results: Sequencing came back and results indicated that the gene if interest, LuxOD47E was not in the psB1AC3 vector.  We will have to go back to the gene in the pCR2.1-TOPO vector and try to get it into the psB1AC3 vector.
 <html>