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| text-align: justify; | | text-align: justify; |
| } | | } |
| + | |
| + | .center { |
| + | text-align: center; |
| + | display: block; |
| + | margin-left: auto; |
| + | margin-right: auto; |
| + | } |
| + | |
| </style> | | </style> |
| | | |
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| <div id="leftmenu"> | | <div id="leftmenu"> |
| <td width="210" rowspan=30 bgcolor="#414141" valign="top"> | | <td width="210" rowspan=30 bgcolor="#414141" valign="top"> |
- | <br><center>
| |
| <br> | | <br> |
| + | <div class="heading">THIS MONTH</div> |
| + | <div class="desc"> |
| + | <center> |
| + | </html> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=05 | title=Team:Calgary}} |
| + | |} |
| + | <html> |
| + | </center> |
| + | </div> |
| <br> | | <br> |
- |
| |
- | <table>
| |
- | <tr>
| |
- | <td>
| |
- | <table class="month">
| |
- |
| |
- | <tr class="heading">
| |
- | <td colspan="7">May
| |
- | </td></tr>
| |
- | <tr class="dow">
| |
- | <td>M</td><td>T</td><td>W</td><td>T</td><td>F</td><td>S</td><td>S
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td>
| |
- | </td><td>
| |
- | </td><td>
| |
- | </td><td>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/1_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/1_May_2009" rel="nofollow"><span class="day-active">1</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/2_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/2_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">2</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/3_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/3_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">3</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/4_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/4_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">4</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/5_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/5_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">5</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/6_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/6_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">6</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/7_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/7_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">7</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/8_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/8_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">8</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/9_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/9_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">9</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/10_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/10_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">10</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/11_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/11_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">11</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/12_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/12_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">12</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/13_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/13_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">13</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/14_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/14_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">14</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/15_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/15_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">15</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/16_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/16_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">16</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/17_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/17_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">17</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/18_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/18_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">18</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/19_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/19_May_2009" rel="nofollow"><span class="day-active">19</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/20_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/20_May_2009" rel="nofollow"><span class="day-active">20</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/21_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/21_May_2009" rel="nofollow"><span class="day-active">21</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/22_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/22_May_2009" rel="nofollow"><span class="day-active">22</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/23_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/23_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">23</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/24_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/24_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">24</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/25_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/25_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">25</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/26_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/26_May_2009" rel="nofollow"><span class="day-active">26</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/27_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/27_May_2009" rel="nofollow"><span class="day-active">27</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/28_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/28_May_2009" rel="nofollow"><span class="day-active">28</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/29_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/29_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">29</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/30_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/30_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">30</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/31_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/31_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">31</span></a>
| |
- | </td></tr></table>
| |
- | </td></tr></table>
| |
- | <p>
| |
- |
| |
- | </center>
| |
| <div class="heading">NOTEBOOK PAGE INDEX</div> | | <div class="heading">NOTEBOOK PAGE INDEX</div> |
| <div class="desc"> | | <div class="desc"> |
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| </div> | | </div> |
| </p></div> | | </p></div> |
| + | <br> |
| + | <br> |
| + | <div class="heading">CALENDAR</div> |
| + | <div class="desc"> |
| + | <center> |
| + | </html> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=05 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=06 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=07 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=08 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=09 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=10 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | <html> |
| + | <center> |
| + | </desc> |
| </td> | | </td> |
| <td width="10" rowspan=30 bgcolor="#222222"> | | <td width="10" rowspan=30 bgcolor="#222222"> |
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| <br> | | <br> |
| <div class="heading"> | | <div class="heading"> |
- | MAY 27, 2009
| + | May 19, 2009 |
| </div> | | </div> |
| <br> | | <br> |
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| <br> | | <br> |
| <div class="heading"> | | <div class="heading"> |
- | Descriptive Title of What You're Doing | + | Descriptive Title of What You’re Doing |
| </div> | | </div> |
| <br> | | <br> |
| <div class="desc"> | | <div class="desc"> |
| </html> | | </html> |
- | WIKI CODING HERE: | + | WIKI CODING HERE |
- | | + | |
- | *bullet
| + | |
- | <br>
| + | |
- | <b>bold</b>
| + | |
- | <i>italics</i>
| + | |
- | <u>underline</u>
| + | |
- | <br>
| + | |
- | # Named link: [http://en.wikipedia.org Wikipedia]
| + | |
- | <br>
| + | |
- | link to books using ISBNs
| + | |
- | <br>
| + | |
- | [[Image:wiki.png]]
| + | |
- | will end up like:<br>
| + | |
- | [[Image:Thane4.png]]
| + | |
- | | + | |
| <html> | | <html> |
| </div> | | </div> |
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| | | |
| </div> | | </div> |
| + | </td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td height="10px" bgcolor="#222222"> |
| </td> | | </td> |
| </tr> | | </tr> |
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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|
CHINMOYEE
Descriptive Title of What You're Doing
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|
EMILY
Descriptive Title of What You're Doing
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Descriptive Title of What You're Doing
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KATIE
Descriptive Title of What You're Doing
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KEVIN
Verification of Pqrr4 for Reporter circuit
Restriction Digest
To verify the isolated Pqrr4, restriction digest was done with NotI (invitrogen, Lot 2803027) enzymes, React Buffer 3, and GeneRuler SM1333 ladder.
Polymerase Chain Reaction (PCR)
The Pqrr4 was then amplified using Polymerase Chain Reaction (PCR) from biobrick AC (pSB1AC3) using primers Pqrr4 F/R and platinum Thermus aquaticus (pTaq; invitrogen) according to the specifications of the manufacturer. The following cycling conditions were used:
94°C for 3 min; 36x (94°C for 30s; 53°C for 45s; 72°C for 1 min;) 72°C for 10 min; held at 4°C.
Sequencing
Lastly, the Colony 1, being Pqrr4 BBK Green, was sent for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada).
Sequencing needs to be added
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Descriptive Title of What You're Doing
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Descriptive Title of What You're Doing
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