|
|
| Line 137: |
Line 137: |
| | <br> | | <br> |
| | <div class="heading"> | | <div class="heading"> |
| - | June 2, 2009
| + | JUNE 2, 2009 |
| | </div> | | </div> |
| | <br> | | <br> |
| Line 157: |
Line 157: |
| | <br> | | <br> |
| | <div class="heading"> | | <div class="heading"> |
| - | Descriptive Title of What You're Doing
| + | Isolation of Plasmids (pSB1AC3 and pSB1AK3)for Cloning |
| | </div> | | </div> |
| | <br> | | <br> |
| | <div class="desc"> | | <div class="desc"> |
| | </html> | | </html> |
| - | WIKI CODING HERE:
| + | * Isolated plasmids (pSB1AK3 and pSB1AC3) from overnight cultures prepared by Thane. |
| - | | + | * Protocol: Used GenElute Plasmid Miniprep Kit (Sigma-Aldrich), lot # 048k6062, used as according to the manufacturers instructions. |
| - | *bullet | + | <Br> |
| - | <br> | + | <Br> |
| - | <b>bold</b> | + | *Results: |
| - | <i>italics</i>
| + | <Br> |
| - | <u>underline</u> | + | <center> |
| - | <br> | + | {| border="1" |
| - | # Named link: [http://en.wikipedia.org Wikipedia]
| + | | '''Plasmid''' || '''260/280''' || '''260/230''' || '''Concentration [ng/μL]''' |
| - | <br> | + | |- |
| - | link to books using ISBNs
| + | | ''psB1AC3 1 || 1.96 || 3.48 || 76.9 |
| - | <br>
| + | |- |
| - | [[Image:wiki.png]]
| + | | ''psB1AC3 2 || 1.93 || 2.29 || 98.1 |
| - | will end up like:<br>
| + | |- |
| - | [[Image:Thane4.png]]
| + | | ''psB1AC3 3 || 1.87|| 1.88|| 48.5 |
| | + | |- |
| | + | | ''psB1AC3 4|| 1.90|| 2.11|| 118.9 |
| | + | |- |
| | + | | ''psB1AK3 1 || 2.05 || 2.42 || 134.4 |
| | + | |- |
| | + | | ''psB1AK3 2 || 1.95 || 2.23 || 55.6 |
| | + | |- |
| | + | | ''psB1AK3 3 || 1.91 || 2.22 || 97.9 |
| | + | |- |
| | + | | ''psB1AK3 4 || 2.03 || 1.92 || 44.5 |
| | + | |} |
| | + | </center> |
| | + | * Set up restriction enzyme digest to ensure that biobrick sites are in the vector. The following are the reactions that were done: |
| | + | Reaction 1: pSB1AC3 - cut with NotI |
| | + | Reaction 2: pSB1AC3 - cut with EcoRI and SpeI |
| | + | Reaction 3: pSB1AC3 - cut with XbaI and PstI |
| | + | Reaction 4: pSB1AC3 - cut with XbaI and SpeI |
| | + | Reaction 5: pSB1AK3 - cut with NotI |
| | + | Reaction 6: pSB1AK3 - cut with EcorI and SpeI |
| | + | Reaction 7: pSB1AK3 - cut with XbaI and PstI |
| | + | Reaction 8: pSB1AK3 - cut with XbaI and SpeI |
| | + | * Incubate reactions at 37<sup>o</sup>C |
| | | | |
| | <html> | | <html> |
|
|
CAROL
Isolation of Plasmids (pSB1AC3 and pSB1AK3)for Cloning
- Isolated plasmids (pSB1AK3 and pSB1AC3) from overnight cultures prepared by Thane.
- Protocol: Used GenElute Plasmid Miniprep Kit (Sigma-Aldrich), lot # 048k6062, used as according to the manufacturers instructions.
| Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
|
| psB1AC3 1 | 1.96 | 3.48 | 76.9
|
| psB1AC3 2 | 1.93 | 2.29 | 98.1
|
| psB1AC3 3 | 1.87 | 1.88 | 48.5
|
| psB1AC3 4 | 1.90 | 2.11 | 118.9
|
| psB1AK3 1 | 2.05 | 2.42 | 134.4
|
| psB1AK3 2 | 1.95 | 2.23 | 55.6
|
| psB1AK3 3 | 1.91 | 2.22 | 97.9
|
| psB1AK3 4 | 2.03 | 1.92 | 44.5
|
- Set up restriction enzyme digest to ensure that biobrick sites are in the vector. The following are the reactions that were done:
Reaction 1: pSB1AC3 - cut with NotI
Reaction 2: pSB1AC3 - cut with EcoRI and SpeI
Reaction 3: pSB1AC3 - cut with XbaI and PstI
Reaction 4: pSB1AC3 - cut with XbaI and SpeI
Reaction 5: pSB1AK3 - cut with NotI
Reaction 6: pSB1AK3 - cut with EcorI and SpeI
Reaction 7: pSB1AK3 - cut with XbaI and PstI
Reaction 8: pSB1AK3 - cut with XbaI and SpeI
- Incubate reactions at 37oC
|
|
|
CHINMOYEE
Descriptive Title of What You're Doing
|
|
|
EMILY
Purification of psB1AC3 & psB1AK3 vectors
- Protocol: Used GenElute Plasmid Miniprep Kit (Sigma-Aldrich), lot # 048k6062, used as according to the manufacturers instructions.
| Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
|
| psB1AC3 1 | 1.96 | 3.48 | 76.9
|
| psB1AC3 2 | 1.93 | 2.29 | 98.1
|
| psB1AC3 3 | 1.87 | 1.88 | 48.5
|
| psB1AC3 4 | 1.90 | 2.11 | 118.9
|
| psB1AK3 1 | 2.05 | 2.42 | 134.4
|
| psB1AK3 2 | 1.95 | 2.23 | 55.6
|
| psB1AK3 3 | 1.91 | 2.22 | 97.9
|
| psB1AK3 4 | 2.03 | 1.92 | 44.5
|
|
|
|
FAHD
Descriptive Title of What You're Doing
|
|
|
IMAN
Descriptive Title of What You're Doing
|
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
|
KATIE
Descriptive Title of What You're Doing
|
|
|
KEVIN
Descriptive of What You're Doing
|
|
|
MANDY
Descriptive Title of What You're Doing
|
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
|
PRIMA
Descriptive Title of What You're Doing
|
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
|
VICKI
Descriptive Title of What You're Doing
|
"
