From 2009.igem.org
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| - | Descriptive Title of What You’re Doing
| + | Analyzing ''E.coli'' Colonies |
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| - | WIKI CODING HERE
| + | * Minimal growth of ''E.Coli'' on LB plates with Kan resistance. There were only 5 colonies found. None of the colonies were glowing. We are not sure what 'glowing' would look like. |
| | + | * Ordered primers that would allow us to clone part BBa_B0040 in front of the luciferase operon. The following is the primers that were ordered. |
| | + | XhoI-R40-F: GCGCTCGAGTCCCTATCAGTGATAGAG |
| | + | BamHI-R40-R: GCGGGATCCGTGCTCAGTATCTCTATC |
| | + | These primers would allow us to attach XhoI and BamHI sites onto part BBa_B0040 by PCR and then we are able to clone this part into pCS26 vector with luciferase as the reporter. This should allow us to see the glowing from the luciferase operon in the plasmid. |
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Revision as of 02:23, 29 July 2009
University of Calgary
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CAROL
Analyzing ''E.coli'' Colonies
- Minimal growth of E.Coli on LB plates with Kan resistance. There were only 5 colonies found. None of the colonies were glowing. We are not sure what 'glowing' would look like.
- Ordered primers that would allow us to clone part BBa_B0040 in front of the luciferase operon. The following is the primers that were ordered.
XhoI-R40-F: GCGCTCGAGTCCCTATCAGTGATAGAG
BamHI-R40-R: GCGGGATCCGTGCTCAGTATCTCTATC
These primers would allow us to attach XhoI and BamHI sites onto part BBa_B0040 by PCR and then we are able to clone this part into pCS26 vector with luciferase as the reporter. This should allow us to see the glowing from the luciferase operon in the plasmid.
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CHINMOYEE
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EMILY
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FAHD
Calling Oil & Gas and Research & Development Pharmaceutical Companies
Following is a list of Research & Development Pharmaceutical Companies I contacted and researched on today:
1) Life Technologies Inc.
2) Charles River Labs
3) Boheringer Ingelheim Canada Ltd.
4) EMD Chemicals
5) Paladin Labs
6) VWR Canada
Following is a list of Oil & Gas Companies I contacted and researched on today:
1) Teck Coal Ltd.
2) Focus Corporation
3) Imperial oil resources
4) Enbridge Pipelines
5) Bietz Resources
6) Bow Valley Energy Ltd.
7) Ithaca Energy Inc.
For today, Boheringer Ingelhiem Canada was the only Research & Development Pharmaceutical Company that said that it might be able to help us. I will do a follow-up on our 2009 iGEM sponsorship proposal next week.
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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