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Team:Calgary/22 July 2009
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* Prepared sterilized LB broth for growing cells. Followed procedure found in the protocol page for making competent cells. | * Prepared sterilized LB broth for growing cells. Followed procedure found in the protocol page for making competent cells. | ||
* After completion of making competent cells, transformed ligated products (pCS26+promoters ligated using NEB Quick Ligase) into XL Gold Ultracompetent cells. These cells were plated on LB+Kan agar plates and was incubated overnight at 37<sup>o</sup>C. | * After completion of making competent cells, transformed ligated products (pCS26+promoters ligated using NEB Quick Ligase) into XL Gold Ultracompetent cells. These cells were plated on LB+Kan agar plates and was incubated overnight at 37<sup>o</sup>C. | ||
| + | * As well, as a control, we transformed pBluescript into both XL Gold Ultracompetent cells and Top 10 Cells and plated cells on LB plates to incubate overnight at 37<sup>o</sup>C. | ||
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Revision as of 04:00, 29 July 2009
UNIVERSITY OF CALGARY
