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| Line 386: |
Line 386: |
| | Lastly, the Colony 1, being <i>Pqrr4</i> BBK Green, was sent for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada). | | Lastly, the Colony 1, being <i>Pqrr4</i> BBK Green, was sent for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada). |
| | <br> | | <br> |
| - | <nowiki>
| |
| | | | |
| - | NNNNNNNNATNANNTATNNN
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| - |
| |
| - | ATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTG
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| - |
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| - | GAATTCGCGGCCGCTTCTAGAGTATCAGCAAAAACACTACGGTGGATAATCAGTAAAACCATGAAACTAGAGCCCCGCACACTTGCGGGGCTTTTTAATTTTGAATTTCTTTCTTATTAAAACGCCATTTTTCTGATAAATGTATTAGTAGCAATGCGCATGGTGGCATATTTGCATCATTTTGCATTTTGCAAATGCGATTTGCAAAATGCGTGCTCAATAAAGCACCAATATGCATCAGGATCGAAGAAAAAAGGCGTTTTTAAAAGTTGGCACGCATCGTGCTTTATACAGATACTAGTAGCGGCCGCTGCAG
| |
| - |
| |
| - | TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGA
| |
| - |
| |
| - | NCGANGTATGTAGGNNNGCTACAGANTTCTTGAGTNNNGCCTACTACGGCTACNCTANANANAGT
| |
| - |
| |
| - | Query: Sequenced Pqrr4
| |
| - | Subject: Expected sequence of Pqrr4
| |
| - |
| |
| - | >lcl|61039
| |
| - | Length=275
| |
| - |
| |
| - | Score = 508 bits (275), Expect = 2e-148
| |
| - | Identities = 275/275 (100%), Gaps = 0/275 (0%)
| |
| - | Strand=Plus/Plus
| |
| - |
| |
| - | Query 89 GTATCAGCAAAAACACTACGGTGGATAATCAGTAAAACCATGAAACTAGAGCCCCGCACA 148
| |
| - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |
| - | Sbjct 1 GTATCAGCAAAAACACTACGGTGGATAATCAGTAAAACCATGAAACTAGAGCCCCGCACA 60
| |
| - |
| |
| - | Query 149 CTTGCGGGGCTTTTTAATTTTGAATTTCTTTCTTATTAAAACGCCATTTTTCTGATAAAT 208
| |
| - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |
| - | Sbjct 61 CTTGCGGGGCTTTTTAATTTTGAATTTCTTTCTTATTAAAACGCCATTTTTCTGATAAAT 120
| |
| - |
| |
| - | Query 209 GTATTAGTAGCAATGCGCATGGTGGCATATTTGCATCATTTTGCATTTTGCAAATGCGAT 268
| |
| - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |
| - | Sbjct 121 GTATTAGTAGCAATGCGCATGGTGGCATATTTGCATCATTTTGCATTTTGCAAATGCGAT 180
| |
| - |
| |
| - | Query 269 TTGCAAAATGCGTGCTCAATAAAGCACCAATATGCATCAGGATCGAAGAAAAAAGGCGTT 328
| |
| - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |
| - | Sbjct 181 TTGCAAAATGCGTGCTCAATAAAGCACCAATATGCATCAGGATCGAAGAAAAAAGGCGTT 240
| |
| - |
| |
| - | Query 329 TTTAAAAGTTGGCACGCATCGTGCTTTATACAGAT 363
| |
| - | |||||||||||||||||||||||||||||||||||
| |
| - | Sbjct 241 TTTAAAAGTTGGCACGCATCGTGCTTTATACAGAT 275
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| - |
| |
| - | </nowiki>
| |
| | <br> | | <br> |
| | <html> | | <html> |
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|
CAROL
Modeling Readings
- Focused on how part BBa_F2620 was characterized and wrote up simple protocols to test our circuit when the circuit is completed.
- Some of the characteristics includes: gene stability, response time, dynamic response and specificity.
- Started reading up on Simbiology and what kind of functionality the platform provides.
- No experiments performed.
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Verification of Pqrr4 for Reporter circuit
Restriction Digest
To verify the isolated Pqrr4, restriction digest was done with NotI (invitrogen, Lot 2803027) enzymes, React Buffer 3, and GeneRuler SM1333 ladder.
Polymerase Chain Reaction (PCR)
The Pqrr4 was then amplified using Polymerase Chain Reaction (PCR) from biobrick AC (pSB1AC3) using primers Pqrr4 F/R and platinum Thermus aquaticus (pTaq; invitrogen) according to the specifications of the manufacturer. The following cycling conditions were used:
94°C for 3 min; 36x (94°C for 30s; 53°C for 45s; 72°C for 1 min;) 72°C for 10 min; held at 4°C.
Sequencing
Lastly, the Colony 1, being Pqrr4 BBK Green, was sent for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada).
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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