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Minnesota/9 June 2009
From 2009.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
! Reverse Kinetic Constant | ! Reverse Kinetic Constant | ||
|- | |- | ||
| - | | RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP||1E+07|| 1 | + | |1/2||RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP||1E+07|| 1 |
|- | |- | ||
| - | |RNA:lacP -> RNAp:lacP* || .01 || | + | |3||RNA:lacP -> RNAp:lacP* || .01 || |
|- | |- | ||
| - | |RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp||30|| | + | |4||RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp||30|| |
|- | |- | ||
| - | |RNAp:DNAgfp -> RNAp + gfp_mRNA||30|| | + | |5||RNAp:DNAgfp -> RNAp + gfp_mRNA||30|| |
|- | |- | ||
| - | |gfp_mRNA + rib -> rib:gfp_mRNA||100000|| | + | |6||gfp_mRNA + rib -> rib:gfp_mRNA||100000|| |
|- | |- | ||
| - | |rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA||33|| | + | |7||rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA||33|| |
|- | |- | ||
| - | |rib:gfp_mRNA_1 -> rib + gfp||33|| | + | |8||rib:gfp_mRNA_1 -> rib + gfp||33|| |
|- | |- | ||
| - | + | |9||gfp_mRNA -> Ø||1.16E-03|| | |
| - | |gfp_mRNA -> Ø||1.16E-03|| | + | |
|- | |- | ||
| - | |gfp -> Ø||3.21E-05|| | + | |10||gfp -> Ø||3.21E-05|| |
|- | |- | ||
| - | |tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04 | + | |11\12||tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04 |
|- | |- | ||
| - | |tetR2:aTc + aTc ↔ tetR2:aTc2||1E+08||1E-03 | + | |13\14||tetR2:aTc + aTc ↔ tetR2:aTc2||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2 + tetO1 ↔ tetR2:tetO1||1E+08||1E-03 | + | |15\16||tetR2 + tetO1 ↔ tetR2:tetO1||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc||1E+08||1 | + | |17\18||tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc||1E+08||1 |
|- | |- | ||
| - | |tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2||1E+08||1E+05 | + | |19\20||tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2||1E+08||1E+05 |
|- | |- | ||
| - | |tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc||1E+08||1E-03 | + | |21\22||tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2||1E+08||1E-03 | + | |23\24||tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2 + tetO2 ↔ tetR2:tetO2||1E+08||1E-03 | + | |25\26||tetR2 + tetO2 ↔ tetR2:tetO2||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc||1E+08||1 | + | |27\28||tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc||1E+08||1 |
|- | |- | ||
| - | |tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc2||1E+08||1E+05 | + | |29\30||tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc2||1E+08||1E+05 |
|- | |- | ||
| - | |tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc||1E+08||1E-03 | + | |31\32||tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc2||1E+08||1E-03 | + | |33\34||tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc2||1E+08||1E-03 |
|- | |- | ||
| - | |tetR2 -> Ø||2.89E-04|| | + | |35||tetR2 -> Ø||2.89E-04|| |
|- | |- | ||
| - | |tetR2:aTc -> aTc||2.89E-04|| | + | |36||tetR2:aTc -> aTc||2.89E-04|| |
|- | |- | ||
| - | |tetR2:aTc2 -> 2 aTc||2.89E-04|| | + | |37||tetR2:aTc2 -> 2 aTc||2.89E-04|| |
|- | |- | ||
| - | |tetR2 + nsDNA ↔ tetR2:nsDNA||1000||3.2409 | + | |38\39||tetR2 + nsDNA ↔ tetR2:nsDNA||1000||3.2409 |
|- | |- | ||
| - | |tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA||1000||3.2409 | + | |40\41||tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA||1000||3.2409 |
|- | |- | ||
| - | |tetR2:aTc:nsDNA -> aTc + nsDNA||1.93E-04|| | + | |42||tetR2:aTc:nsDNA -> aTc + nsDNA||1.93E-04|| |
|- | |- | ||
| - | |tetR2:nsDNA -> nsDNA||1.93E-04|| | + | |43||tetR2:nsDNA -> nsDNA||1.93E-04|| |
|- | |- | ||
| - | |Ø -> tetR2||1E-11|| | + | |44||Ø -> tetR2||1E-11|| |
|- | |- | ||
| - | |Ø -> aTc||3.3E-04|| | + | |45||Ø -> aTc||3.3E-04|| |
|} | |} | ||
Revision as of 18:57, 29 July 2009
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Today Patrick arrived and began working on getting up to speed on the team's TTL model and creating the TTN model he will eventually be working on.
The model created was similar to that described previously except with the addition of a second tet operon (tetO2):
| Rxn # | Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
|---|---|---|---|
| 1/2 | RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP | 1E+07 | 1 |
| 3 | RNA:lacP -> RNAp:lacP* | .01 | |
| 4 | RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp | 30 | |
| 5 | RNAp:DNAgfp -> RNAp + gfp_mRNA | 30 | |
| 6 | gfp_mRNA + rib -> rib:gfp_mRNA | 100000 | |
| 7 | rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA | 33 | |
| 8 | rib:gfp_mRNA_1 -> rib + gfp | 33 | |
| 9 | gfp_mRNA -> Ø | 1.16E-03 | |
| 10 | gfp -> Ø | 3.21E-05 | |
| 11\12 | tetR2 + aTc ↔ tetR2:aTc | 2E+09 | 4E-04 |
| 13\14 | tetR2:aTc + aTc ↔ tetR2:aTc2 | 1E+08 | 1E-03 |
| 15\16 | tetR2 + tetO1 ↔ tetR2:tetO1 | 1E+08 | 1E-03 |
| 17\18 | tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc | 1E+08 | 1 |
| 19\20 | tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E+05 |
| 21\22 | tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc | 1E+08 | 1E-03 |
| 23\24 | tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E-03 |
| 25\26 | tetR2 + tetO2 ↔ tetR2:tetO2 | 1E+08 | 1E-03 |
| 27\28 | tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc | 1E+08 | 1 |
| 29\30 | tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc2 | 1E+08 | 1E+05 |
| 31\32 | tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc | 1E+08 | 1E-03 |
| 33\34 | tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc2 | 1E+08 | 1E-03 |
| 35 | tetR2 -> Ø | 2.89E-04 | |
| 36 | tetR2:aTc -> aTc | 2.89E-04 | |
| 37 | tetR2:aTc2 -> 2 aTc | 2.89E-04 | |
| 38\39 | tetR2 + nsDNA ↔ tetR2:nsDNA | 1000 | 3.2409 |
| 40\41 | tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA | 1000 | 3.2409 |
| 42 | tetR2:aTc:nsDNA -> aTc + nsDNA | 1.93E-04 | |
| 43 | tetR2:nsDNA -> nsDNA | 1.93E-04 | |
| 44 | Ø -> tetR2 | 1E-11 | |
| 45 | Ø -> aTc | 3.3E-04 |
