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Team:Calgary/5 June 2009
From 2009.igem.org
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Competent TOP10 cells are regularly required in our labs because they are used to transform most of our plasmids. In order to make TOP10 cells competent, we need to go through several cycles of heat and cold shocking them, and exposing them to CaCl<sub>2</sub>. This pokes holes in their membrane, which allows plasmids to move through. These competent cells were then verified via transformation of pBluescript, which are very small and easy for cells to uptake. | Competent TOP10 cells are regularly required in our labs because they are used to transform most of our plasmids. In order to make TOP10 cells competent, we need to go through several cycles of heat and cold shocking them, and exposing them to CaCl<sub>2</sub>. This pokes holes in their membrane, which allows plasmids to move through. These competent cells were then verified via transformation of pBluescript, which are very small and easy for cells to uptake. | ||
<br><br> | <br><br> | ||
| - | Figure 1. Verification of competent cells via transformation of pBluescript | + | Figure 1. Verification of competent cells via transformation of pBluescript<br> |
[[Image:Calgary_2009.06.06.Comp.Cell.Verif.png]] | [[Image:Calgary_2009.06.06.Comp.Cell.Verif.png]] | ||
<b>Preparation of the campus fair</b><br> | <b>Preparation of the campus fair</b><br> | ||
Revision as of 20:01, 30 July 2009
UNIVERSITY OF CALGARY
