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Minnesota/30 June 2009
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(Difference between revisions)
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'''Ben'''<br> | '''Ben'''<br> | ||
| + | {| class="wikitable" style="text-align:center" border = "1" | ||
| + | |- | ||
| + | ! Reaction | ||
| + | ! Forward Kinetic Constant | ||
| + | ! Reverse Kinetic Constant | ||
| + | |- | ||
| + | |RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |- | ||
| + | |RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |- | ||
| + | ||RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |} | ||
[[Image:Tnnatcvariesleaky11.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> | [[Image:Tnnatcvariesleaky11.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> | ||
| + | These graphs show almost no change from yesterday's graph, even though the kinetic constant has been reduced by an order of magnitude. This is probably due to the fact that the constant is so low already, and so making it even lower will have little effect. | ||
Latest revision as of 16:21, 25 August 2009
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Patrick
Here is the "leaky" graph along with the base for comparison:
 Figure 1 - Base TTN Model |
 Figure 2 - Leaky Model |
As can be seen the "leaky" graph indeed does show increased GFP production at low aTc concentrations.
Ben
| Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
|---|---|---|
| RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 | |
| RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 | |
| RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 |
These graphs show almost no change from yesterday's graph, even though the kinetic constant has been reduced by an order of magnitude. This is probably due to the fact that the constant is so low already, and so making it even lower will have little effect.
