Template:Team:KULeuven/30 July 2009/VanillinProduction

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(Difference between revisions)
(New page: following parts were put in competent cells by electroporation: * ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}} * fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}} the cells were the...)
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following parts were put in competent cells by electroporation:
following parts were put in competent cells by electroporation:
-
* ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}}
+
*RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}}
-
* fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}}
+
*RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}}
the cells were then put in liquid LB medium to recuperate from the procedure. after a few hours they were transfered on LB/amp plates and grown overnight at 37°C.
the cells were then put in liquid LB medium to recuperate from the procedure. after a few hours they were transfered on LB/amp plates and grown overnight at 37°C.

Revision as of 07:28, 31 July 2009

following parts were put in competent cells by electroporation:

  • RBS + ech, in pSB1A2,
  • RBS + fcs, in pSB1A2,

the cells were then put in liquid LB medium to recuperate from the procedure. after a few hours they were transfered on LB/amp plates and grown overnight at 37°C.