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Team:Calgary/31 July 2009
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* From the overnight digest of R0040 with XhoI and BamHI, the promoter was ligated with vector, pCS26. | * From the overnight digest of R0040 with XhoI and BamHI, the promoter was ligated with vector, pCS26. | ||
* These plasmids were transformed into XL Gold cells and was plated on Kanamycin plates overnight. | * These plasmids were transformed into XL Gold cells and was plated on Kanamycin plates overnight. | ||
| - | + | Team meeting: | |
| - | + | * Lab:discussed about promoter library and the failure to see the expression of luciferase. Also, discussed about the primer design of R0040, so that we can clone the constitutively ON promoter in front of luciferase. This will show us what the luciferase expression will look like. | |
| - | * Lab: | + | |
* Modelling: Discussed about Robustness. The three things that we decided to look at for robustness is: 1. PQ expression (the selection of the 'best' sigma 70 promoter) 2. Temperature Alterations (to see if changes in temperature (from 37<sup>o</sup>C to another temperature would affect expression) 3. Type of media (LB vs. LM) | * Modelling: Discussed about Robustness. The three things that we decided to look at for robustness is: 1. PQ expression (the selection of the 'best' sigma 70 promoter) 2. Temperature Alterations (to see if changes in temperature (from 37<sup>o</sup>C to another temperature would affect expression) 3. Type of media (LB vs. LM) | ||
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Revision as of 18:36, 31 July 2009
UNIVERSITY OF CALGARY
