Team:Calgary/13 July 2009
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- | + | *Today I started with re-running my gel from Friday from the PCR products in the freezer. See gel photo below. | |
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+ | *Analysis: Lanes 1-4 are J13002-LuxOD47E-B0015 trial 1 (with the J13002-LuxOD47E construct as the insert) and Lanes 5-8 are J13002-LuxOD47E-B0015 trial 2 (with B0015 as the insert). Lane 9 is a size control with J13002-LuxOD47E and Lane 10 is a size control with only J13002. If our transformation was successful, we expect to see lanes 1-8 slightly higher then lane 9, which should be slightly higher than lane 10. | ||
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+ | *Looking at this gel, we do not see a size difference at all between lanes 1-8 and lane 9. This indicates that either our transformation was not successful or our initial contruction digest was not successful. Because we have all ready gone back to the unligated product once with no results, we will try this again going back to contruction. | ||
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+ | *Today I also made restreaks of the J13002-Lux0D47E constructs on KT1144 plates as well as overnight cultures of J13002-LuxOD47E and LuxOD47E witht he purpose of making glycerol stocks tomorrow. | ||
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Revision as of 21:07, 17 August 2009
UNIVERSITY OF CALGARY