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Team:Virginia/4 August 2009
From 2009.igem.org
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*New PCR to HA to ABP again | *New PCR to HA to ABP again | ||
| - | **Temps: | + | **Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C) |
Latest revision as of 18:56, 4 August 2009
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Today
- Miniprep of ABP / ABP+HA in vectors
- Conc: 17.8ng/ul and 33.3ng/ul, respectively
- Digest of above (E,P)
- Gel:
- New PCR to HA to ABP again
- Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C)
