Team:KULeuven/Used Parts

From 2009.igem.org

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| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || -80 ||  
| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || -80 ||  
|-align="center"
|-align="center"
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| {{kulpart|BBa_I742106|icon}}|| Sam8 + RBS || A || send from Edinburgh || -20 ||   
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| {{kulpart|BBa_I742106|icon}}|| RBS + Sam8 || A || send from Edinburgh || -20 ||   
|-align="center"
|-align="center"
| {{kulpart|BBa_K238007|icon}}|| Sam5 || A || send from Edinburgh || -20 ||
| {{kulpart|BBa_K238007|icon}}|| Sam5 || A || send from Edinburgh || -20 ||

Revision as of 12:36, 5 August 2009

Individual parts

PartnumberShort description Resistance StatusLocationAdditional notes
Ribokey A/A ordered at GeneArt
antikey A/A ordered at GeneArt
lock Ap ordered at GeneArt
Terminator Ak Available here -80
RBS Ap Available here -80
promotor Ap Available here -80
RBS + Sam8 A send from Edinburgh -20
Sam5 A send from Edinburgh -20
comt A/A send from Edinburgh/available in iGEM plates -20
fcs Ap received from Edinburgh -20
ech Ap received from Edinburgh -20
VirA receptor primers ordered This is purified from Agrobacterium temefaciens
VirG activator primers ordered This is purified from Agrobacterium tumefaciens
RpoA primers ordered This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB promoter region primers ordered This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
YcgF/E promotor region purified from E.coli strain MC4100 -20 blue light promotor region from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1
YcgF/E promotor primers ordered exact blue light promotor from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1

Composite parts

PartnumberShort description Resistance StatusLocationAdditional notes
VirB promotor + key Biobrick in registry
Blr promotor + key Biobrick in registry
Vanillin receptor Biobrick in registry
Vanillin synthesis Biobrick in registry 5 paths in pathway never tested together in E. coli
Short Vanillin synthesis Biobrick in registry Short version containing the last 2 genes of the pathway, these have been tested in E. coli
Short Vanillin synthesis II Biobrick in registry Short version containing the first 3 parts of the pathway, these have not been tested in E. coli
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