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Team:Calgary/5 August 2009
From 2009.igem.org
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| - | + | 1. Plasmid Isolation of <i>Pqrr4</i>+B0034+K082003</div> | |
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| - | + | <i>Pqrr4</i>+B0034+K082003 was isolated in order to verify its presence using Restriction digest and Sequencing later on. The product's purity and concentration were measured using Nanodrop utility and spectrophotometer. | |
| + | {| border="1" | ||
| + | | '''Plasmid''' || '''260/280''' || '''260/230''' || '''Concentration [ng/μL]''' | ||
| + | |- | ||
| + | | ''Pqrr4''+B0034+K082003 C9 #1|| 2.02 || 2.72 || 158.0 | ||
| + | |-| ''Pqrr4''+B0034+K082003 C9 #2|| 2.06 || 3.61 ||160.4 | ||
| + | |} | ||
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| + | 2. Restriction digest of <i>Pqrr4</i>+B0034+K082003</div> | ||
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| + | Restriction digest on <i>Pqrr4</i>+B0034+K082003 to verify its presence. This is our reporter circuit. The product was ran on 1.5% gel at 90V. | ||
[[Image:Calgary_2009.08.05.Pqrr4%2BB0034%2BK082003.png|700px]] | [[Image:Calgary_2009.08.05.Pqrr4%2BB0034%2BK082003.png|700px]] | ||
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| + | 3. Plasmid Switch of <i>Pqrr4</i>+I13500</div> | ||
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| + | Yesterday, I have done an overnight digestion of <i>Pqrr4</i>+I13500 and Q04510 (inverter) part in pSB2K3 vector. Today, with this product, I have performed phophatase treatment on Q04510 and ligated them together. This product was then transformed into TOP10 cells, and is now growing in the incubator at 37˚C. | ||
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Revision as of 21:51, 5 August 2009
UNIVERSITY OF CALGARY
