Team:Calgary/7 July 2009
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The ligated construct of J13002 and GFP/RFP were transformed into TOP10 cells. This was done in order to test the functionality of the fluorescent proteins and the promoter. A brightest colony of each were then restreaked. The following is an image of the restreak: | The ligated construct of J13002 and GFP/RFP were transformed into TOP10 cells. This was done in order to test the functionality of the fluorescent proteins and the promoter. A brightest colony of each were then restreaked. The following is an image of the restreak: | ||
<br> | <br> | ||
+ | <center> | ||
[[Image:Calgary_2009.07.10.I13502_E0040_J13002.png|500px]] | [[Image:Calgary_2009.07.10.I13502_E0040_J13002.png|500px]] | ||
+ | </center> | ||
<br><br> | <br><br> | ||
From it, one can clearly see that RFP is glowing much brighter than the negative control, and that GFP is, although less obvious, also glowing. | From it, one can clearly see that RFP is glowing much brighter than the negative control, and that GFP is, although less obvious, also glowing. |
Revision as of 18:53, 6 August 2009
UNIVERSITY OF CALGARY