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Template:Team:KULeuven/7 August 2009/VanillinReceptor
From 2009.igem.org
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(New page: * primers for mutagenesis have arrived * We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used this frage...) |
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* primers for mutagenesis have arrived | * primers for mutagenesis have arrived | ||
| - | * We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used | + | * We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used these fragments. |
* LBmedium was made with X-gal to select cells afterwards. | * LBmedium was made with X-gal to select cells afterwards. | ||
* Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium. | * Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium. | ||
Revision as of 11:52, 10 August 2009
- primers for mutagenesis have arrived
- We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used these fragments.
- LBmedium was made with X-gal to select cells afterwards.
- Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium.
