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Team:USTC/Notebook/Lab Work/9 August 2009
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Cherrytree (Talk | contribs) (New page: {{:Team:USTC/Template/Header}} {{:Team:USTC/Notebook/Lab_Work}} == {{SUBPAGENAME}} == * The work has almost been finished! The preliminary PCR-primer biobricks have been designed, and we w...) |
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* The work has almost been finished! The preliminary PCR-primer biobricks have been designed, and we will choose 7 better ones from 15~20 primers to synthesize. | * The work has almost been finished! The preliminary PCR-primer biobricks have been designed, and we will choose 7 better ones from 15~20 primers to synthesize. | ||
--[[User:Cherrytree|Cherrytree]] 15:24, 9 August 2009 (UTC) | --[[User:Cherrytree|Cherrytree]] 15:24, 9 August 2009 (UTC) | ||
| + | |||
| + | ---- | ||
| + | *K176036 in pSB1A3: correct but 1-45bp overlap peaks and the counting-backwards 50bps overlap peaks. | ||
| + | *K176036 in pSB1A3: correct. | ||
| + | *K176035 in pSB1A3: incorrect, G1344A in ccdB mutated. | ||
| + | *K176035 in pSB1A3: correct but the counting-backwards 50bps are unclear. | ||
| + | *K176079 in pSB1A3: result shows that it is K176075, 1-936bp correct. | ||
| + | *K176071 in pSB1A3: 1-936bp incorrect. C143A, G678T, in tetR mutated. | ||
| + | *K176075 in pSB1A3: result shows that it is K176079. 1-70bp correct. overlap peaks after that. Perhaps there are two kinds of plasmid. | ||
Latest revision as of 05:45, 1 October 2009
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Team:USTC/Notebook/Lab Work/9 August 2009
Contents |
We chose to use [http://ipadeln.com/ iPad] as our Electronic Lab Notebook. iPad is recommended on OWW [http://openwetware.org/wiki/OpenWetWare:Software/Projects/Lab_Notebooks/Technology].
Our work progress is also updated here.
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Team:USTC/Notebook/Lab Work/9 August 2009
- The work has almost been finished! The preliminary PCR-primer biobricks have been designed, and we will choose 7 better ones from 15~20 primers to synthesize.
--Cherrytree 15:24, 9 August 2009 (UTC)
- K176036 in pSB1A3: correct but 1-45bp overlap peaks and the counting-backwards 50bps overlap peaks.
- K176036 in pSB1A3: correct.
- K176035 in pSB1A3: incorrect, G1344A in ccdB mutated.
- K176035 in pSB1A3: correct but the counting-backwards 50bps are unclear.
- K176079 in pSB1A3: result shows that it is K176075, 1-936bp correct.
- K176071 in pSB1A3: 1-936bp incorrect. C143A, G678T, in tetR mutated.
- K176075 in pSB1A3: result shows that it is K176079. 1-70bp correct. overlap peaks after that. Perhaps there are two kinds of plasmid.
