LacI-Latch Notebook

Week 1: Research and Planning 08/06/09 - 12/06/09

Day 1 Monday (08/06/09)

• Researching the Registry for Biobricks
  • Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.

Day 2 Tuesday (09/06/09)

• Researching the Registry for Plasmids.
  • Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.

Day 3 Wednesday (10/06/09)

• Literature search for Parameters of T7 promoter and the Holin system.
  • Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
  • Identified the novagen plasmids pLysS and pLysE as possible solutions.

• Researching the Registry for LacI Repressor and T7 Polymerase
  • Identified K145014, K145001, I732100 for further discusion

Day 4 Thursday (11/06/09)

• Parameter search for the Modeling effort.

Day 5 Friday (12/06/09)

• End of week meeting
• Discussion and planning of following week

Week 2: BioBrick Rescue 15/06/09 - 19/06/09

Day 1 Monday (15/06/09)

• Prepared LB medium for Lab
• Planning of LacI-Latch
• Inoculated J23100, K145001, B0034 for Miniprep the following day

Day 2 Tuesday (16/06/09)

• Miniprep of J23100, K145001, B0034
• Restriction Digest

Day 3 Wednesday (17/06/09)

• Lambda Digest with HindIII for use as a Ladder
• Gel Electrophoresis of B0034, K145001, J23100, I732100.

Day 4 Thursday (18/06/09)

• Gel Electrophoresis of C0051, R0062, C0040, J37033, K145001, I732100.
• Innoculation of C0051, R0062, J37033, I732100 or miniprep.

Day 5 Friday (19/06/09)

• Miniprep of C0051, R0062, J37033, I732100.

Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09

Day 1 Monday (22/06/09)

• Rescued BBa_1732820, S03518, B0030

Day 2 Tuesday (23/06/09)

Risat:

• Research for Plasmids and J series Promoters
• Rescued and Transformed Psb4k4 and Psb3K5.
• Restriction Digest of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,

Daniel:

• Edinburgh Igem Meeting

Day 3 Wednesday (24/06/09)

Risat:

• Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,

Daniel:

• Edinburgh Igem Meeting

Day 4 Thursday (25/06/09)

Risat:

• Gel Electrophoresis of K112808, K112022, I732100

Daniel:

• Edinburgh Igem Meeting

Day 5 Friday (26/06/09)

• Restriction Digest of E0840
• Gel Electrophoresis of E0840

Week 4 29/06/09 - 03/07/09

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09

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