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Template:Team:KULeuven/3 August 2009/VanillinProduction
From 2009.igem.org
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We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately, the tube containing the Sam5 + RBS (with the PstI site) had opened during transport and all DNA in it was lost. The others were put in competent cells by electroporation. | We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately, the tube containing the Sam5 + RBS (with the PstI site) had opened during transport and all DNA in it was lost. The others were put in competent cells by electroporation. | ||
| - | * Sam5 + RBS in pSB1A2 (with PstI site removed), {{kulpart|BBa_K238007}} | + | * Sam5 + RBS in {{kulpart|pSB1A2}} (with PstI site removed), {{kulpart|BBa_K238007}} |
| - | * Sam8 + RBS in pSB1A2, {{kulpart|BBa_I742106}} | + | * Sam8 + RBS in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742106}} |
| - | * Sam8 in pSB1A2 coding sequence, {{kulpart|BBa_I742142}} | + | * Sam8 in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742142}} |
| - | * ComT in pSB1A2 coding sequence, {{kulpart|BBa_I742107}} | + | * ComT in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742107}} |
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C. | The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C. | ||
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so that we can miniprep them tomorrow. | We also made liquid cultures of the RBS + fcs and RBS + ech cultures so that we can miniprep them tomorrow. | ||
Revision as of 12:35, 1 September 2009
We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately, the tube containing the Sam5 + RBS (with the PstI site) had opened during transport and all DNA in it was lost. The others were put in competent cells by electroporation.
- Sam5 + RBS in (with PstI site removed),
- Sam8 + RBS in ,
- Sam8 in coding sequence,
- ComT in coding sequence,
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so that we can miniprep them tomorrow.
