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Team:KULeuven/Primers
From 2009.igem.org
(Difference between revisions)
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|2261||BLR promoter exact rv||CTG CAG CGG CCG CTA CTA GTA AAA AAT GTT AAT CAA TGT TAA GTG AAG||48||49.6||it has a suffix (not in Tm included) | |2261||BLR promoter exact rv||CTG CAG CGG CCG CTA CTA GTA AAA AAT GTT AAT CAA TGT TAA GTG AAG||48||49.6||it has a suffix (not in Tm included) | ||
| + | |- | ||
| + | |2235||Vanilline receptor Vir A fwd||CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA ACG GAA GAT ATT CAC C||46||67||it has a prefix | ||
| + | |- | ||
| + | |2238||Vanilline receptor Vir A rev||CTG CAG CGG CCG CTA CTA GTA TTA TCA CGT CTT GAT TTT GGT TAG||45||66.1||it has a suffix | ||
| + | |- | ||
| + | |2242||Vanilline receptor Vir G fwd||CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA TTG TAC ATC CTT CAC GTG||48||67.6||it has a prefix | ||
| + | |- | ||
| + | |2243||Vanilline receptor Vir G rev||CTG CAG CGG CCG CTA CTA GTA TTA TCA GGC TGC TGC CAT CGT C||40||69.2||it has a suffix | ||
| + | |- | ||
| + | |2244||Vanilline receptor Rpo A fwd||CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA TTC AGA AGA ACT GGC AG||47||67.9||it has a prefix | ||
| + | |- | ||
| + | |2245||Vanilline receptor Rpo A rev||CTG CAG CGG CCG CTA CTA GTA TTA TTA GTA TTG GTC TTC GTA ACG C||46||66.5||it has a suffix | ||
| + | |- | ||
| + | |2246||Vanilline receptor vir B promoter regio fwd||CAT CAT GAA TTC GCG GCC GCT TCT AGA GTT CGT TCT GAG GCC GAC CTG||48||70.7||it has a prefix | ||
| + | |- | ||
| + | |2247||Vanilline receptor vir B promoter regio rev||CTG CAG CGG CCG CTA CTA GTA TCT CCT TAG CTC GCA ACT AAC ACC G||46||69.9||it has a suffix | ||
| + | |- | ||
| + | |2292||Vanilline receptor mutagenese Rpo A fwd||GTC CTG CAC GAA TTT TCC TCC ATC CCC GGC||30||62||EcoR1 op positie 202 | ||
| + | |- | ||
| + | |2293||Vanilline receptor mutagenese Rpo A rev||GCC GGG GAT GGA GGA AAA TTC GTG CAG GAC||30||62||EcoR1 op positie 202 | ||
| + | |- | ||
| + | |2294||Vanilline receptor mutagenese Rpo A fwd||GAA ATC CGC ATG GAA TTT ACC GTC AAC AAC GGC||33||64.1||EcoR1 op positie 427 | ||
| + | |- | ||
| + | |2295||Vanilline receptor mutagenese Rpo A rev||GCC GTT GTT GAC GGT AAA TTC CAT GCG GAT TTC||33||64.1||EcoR1 op positie 427 | ||
| + | |- | ||
| + | |2288||Vanilline receptor mutagenese vir A fwd||GAC AGC GCC TCA CTG CAA CGC GAT GTA CTC C||31||68||PstI op positie 184 | ||
| + | |- | ||
| + | |2289||Vanilline receptor mutagenese vir A rev||GGA GTA CAT CGC GTT GCA GTG AGG CGC TGT C||31||68||PstI op positie 184 | ||
| + | |- | ||
| + | |2290||Vanilline receptor mutagenese vir A fwd||ATT GCG GAG ATG CTG CAA CGC GAG TGT TTG G||31||67.2||PstI op positie 718 | ||
| + | |- | ||
| + | |2291||Vanilline receptor mutagenese vir A rev||CCA AAC ACT CGC GTT GCA GCA TCT CCG CAA T||31||67.2||PstI op positie 718 | ||
|} | |} | ||
Latest revision as of 09:58, 12 August 2009
| Number | Name | Sequence | Length | Tm (°C) | Comments |
|---|---|---|---|---|---|
| 2171 | BLR promoter large fw | CATCAT GAATTCGCGGCCGCTTCTAGAG TTT GAC AGG TTC GTC GTC | 46 | 52.4 | it has a catcat and prefix (not in Tm included) |
| 2172 | BLR promoter large rv | CTG CAG CGG CCG CTACTAGTA CCT CTG TTA AAA ATG TTA ATC AAT GTT AAG | 51 | 52.1 | it has a suffix (not in Tm included) |
| 2260 | BLR promoter exact fw | CAT CAT GAA TTC GCG GCC GCT TCT AGA GAT TGC AAA AAA TTA ATT TAT CAT TCT G | 55 | 48.6 | it has a catcat and prefix (not in Tm included) |
| 2261 | BLR promoter exact rv | CTG CAG CGG CCG CTA CTA GTA AAA AAT GTT AAT CAA TGT TAA GTG AAG | 48 | 49.6 | it has a suffix (not in Tm included) |
| 2235 | Vanilline receptor Vir A fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA ACG GAA GAT ATT CAC C | 46 | 67 | it has a prefix |
| 2238 | Vanilline receptor Vir A rev | CTG CAG CGG CCG CTA CTA GTA TTA TCA CGT CTT GAT TTT GGT TAG | 45 | 66.1 | it has a suffix |
| 2242 | Vanilline receptor Vir G fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA TTG TAC ATC CTT CAC GTG | 48 | 67.6 | it has a prefix |
| 2243 | Vanilline receptor Vir G rev | CTG CAG CGG CCG CTA CTA GTA TTA TCA GGC TGC TGC CAT CGT C | 40 | 69.2 | it has a suffix |
| 2244 | Vanilline receptor Rpo A fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA TTC AGA AGA ACT GGC AG | 47 | 67.9 | it has a prefix |
| 2245 | Vanilline receptor Rpo A rev | CTG CAG CGG CCG CTA CTA GTA TTA TTA GTA TTG GTC TTC GTA ACG C | 46 | 66.5 | it has a suffix |
| 2246 | Vanilline receptor vir B promoter regio fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA GTT CGT TCT GAG GCC GAC CTG | 48 | 70.7 | it has a prefix |
| 2247 | Vanilline receptor vir B promoter regio rev | CTG CAG CGG CCG CTA CTA GTA TCT CCT TAG CTC GCA ACT AAC ACC G | 46 | 69.9 | it has a suffix |
| 2292 | Vanilline receptor mutagenese Rpo A fwd | GTC CTG CAC GAA TTT TCC TCC ATC CCC GGC | 30 | 62 | EcoR1 op positie 202 |
| 2293 | Vanilline receptor mutagenese Rpo A rev | GCC GGG GAT GGA GGA AAA TTC GTG CAG GAC | 30 | 62 | EcoR1 op positie 202 |
| 2294 | Vanilline receptor mutagenese Rpo A fwd | GAA ATC CGC ATG GAA TTT ACC GTC AAC AAC GGC | 33 | 64.1 | EcoR1 op positie 427 |
| 2295 | Vanilline receptor mutagenese Rpo A rev | GCC GTT GTT GAC GGT AAA TTC CAT GCG GAT TTC | 33 | 64.1 | EcoR1 op positie 427 |
| 2288 | Vanilline receptor mutagenese vir A fwd | GAC AGC GCC TCA CTG CAA CGC GAT GTA CTC C | 31 | 68 | PstI op positie 184 |
| 2289 | Vanilline receptor mutagenese vir A rev | GGA GTA CAT CGC GTT GCA GTG AGG CGC TGT C | 31 | 68 | PstI op positie 184 |
| 2290 | Vanilline receptor mutagenese vir A fwd | ATT GCG GAG ATG CTG CAA CGC GAG TGT TTG G | 31 | 67.2 | PstI op positie 718 |
| 2291 | Vanilline receptor mutagenese vir A rev | CCA AAC ACT CGC GTT GCA GCA TCT CCG CAA T | 31 | 67.2 | PstI op positie 718 |
