August/14 August 2009

From 2009.igem.org

(Difference between revisions)
(miniprep)
(miniprep)
Line 16: Line 16:
   1-3    16.1      1.78  2.40
   1-3    16.1      1.78  2.40
   1-8    14.4      1.77  2.38
   1-8    14.4      1.77  2.38
 +
 +
===transformation===
 +
to get new plasmid
 +
 +
    sample No.
 +
    ③ ④ ⑤ ⑥

Revision as of 23:45, 15 August 2009

miniprep

Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]

  1-12C  29.5ng/μL  1.56  0.91
  1-6I   7.5        1.88  2.79
  1-12A  11.5       1.92  2.65
  1-18L  28.6       1.81  2.46
  1-2M   10.1       1.96  2.60
  1-16G  14.0       1.99  2.47
  2-8I   15.4       1.86  3.29
  2-21F  17.9       1.99  2.60
  2-11N  11.2       1.88  2.32
  2-24G  19.5       1.97  2.34
  2-8O   11.0       1.60  1.74
  2-6E   5.5        1.46  2.03
  1-3    16.1       1.78  2.40
  1-8    14.4       1.77  2.38

transformation

to get new plasmid

   sample No.
    ③ ④ ⑤ ⑥