August/14 August 2009

From 2009.igem.org

(Difference between revisions)
(→miniprep)
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Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]
Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]
-
   1-12C 29.5ng/μL　 1.56  0.91
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C)  29.5ng/μL　 1.56  0.91
-
   1-6I   7.5        1.88  2.79
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I)    7.5        1.88  2.79
-
   1-12A 11.5      1.92  2.65
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A)  11.5      1.92  2.65
-
   1-18L 28.6      1.81  2.46
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L)  28.6      1.81  2.46
-
   1-2M   10.1      1.96  2.60
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M)    10.1      1.96  2.60
-
   1-16G 14.0      1.99  2.47
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G)  14.0      1.99  2.47
-
   2-8I   15.4      1.86  3.29
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I)    15.4      1.86  3.29
-
   2-21F 17.9      1.99  2.60
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F)  17.9      1.99  2.60
-
   2-11N 11.2      1.88  2.32
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N)  11.2      1.88  2.32
-
   2-24G 19.5      1.97  2.34
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G)  19.5      1.97  2.34
-
   2-8O   11.0      1.60  1.74
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O)    11.0      1.60  1.74
-
   2-6E   5.5        1.46  2.03
+
   [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E)    5.5        1.46  2.03
-
   1-3    16.1      1.78  2.40
+
   K204001 - 3    16.1      1.78  2.40
-
   1-8    14.4      1.77  2.38
+
   K204001 - 8    14.4      1.77  2.38
===transformation===
===transformation===

Revision as of 08:55, 21 August 2009

miniprep

Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230] 

  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C)   29.5ng/μL　 1.56  0.91
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I)     7.5        1.88  2.79
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A)   11.5       1.92  2.65
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L)   28.6       1.81  2.46
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M)    10.1       1.96  2.60
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G)   14.0       1.99  2.47
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I)     15.4       1.86  3.29
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F)   17.9       1.99  2.60
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N)   11.2       1.88  2.32
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G)   19.5       1.97  2.34
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O)    11.0       1.60  1.74
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E)     5.5        1.46  2.03
  K204001 - 3    16.1       1.78  2.40
  K204001 - 8    14.4       1.77  2.38

transformation

to get new plasmid 

   sample No.
    ③　④　⑤　⑥
Retrieved from "http://2009.igem.org/August/14_August_2009"