From 2009.igem.org
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| - | Descriptive Title of What You're Doing
| + | Phosphatase Treatment |
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| - | WIKI CODING HERE
| + | I shadowed Carol today. She let me do the phosphatase treatment. |
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| | + | Purpose: Adding phosphatase to her vector. (at this point i didn't know much about her project or what she was doing. I just focused on the techniques). Next step is to ligate the insert and the vector. Finally, we transformed the plasmid into top 10 cells and plated it. |
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| | + | I continued to follow up with the old companies. I mostly left voicemails to the company staff who were away from the desk. I sent our sponsorship packages out to companies who expressed interest in our project. |
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Revision as of 08:21, 21 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Descriptive Title of What You're Doing
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EMILY
Colony PCR for Verification of Construction
- Did a plasmid Isolation of Colony2X LuxOD47E BBK.
- Today I did a colony PCR with p Taq and Biobrick primers (BBK-CP-F and BBK-CP-R) to see if we were able to clone in either the J13002 promoter or the B0015 terminator to our gene of interest, LuxOD47E. PCR products were run on a 1% agarose gel with LuxOD47E BBK as a size control and ddH2O as a negatve control. Gel if pictures below. Lanes 1-4 are J13002-LuxOD47E and Lanes 5-8 are LuxOD47E-B0015. Lane 9 is LuxOD47E BBK, lanes 10 and 11 are left blank and Lane 12 is a negative control with ddH2O.
- From this gel it looks like the cloning of J13002 may have been successful as the band sin the first four lanes appear slighlty higher than the band in the size control lane. We will make overnight cultures of these colonies, isolate plasmid and send the colony with the best concentration for DNA sequencing.
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Descriptive Title of What You're Doing
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KATIE
Descriptive Title of What You're Doing
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KEVIN
Transformation
The ligated construct of J13002 and GFP/RFP were transformed into TOP10 cells. This was done in order to test the functionality of the fluorescent proteins and the promoter. These have to grow overnight.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Phosphatase Treatment
I shadowed Carol today. She let me do the phosphatase treatment.
Purpose: Adding phosphatase to her vector. (at this point i didn't know much about her project or what she was doing. I just focused on the techniques). Next step is to ligate the insert and the vector. Finally, we transformed the plasmid into top 10 cells and plated it.
I continued to follow up with the old companies. I mostly left voicemails to the company staff who were away from the desk. I sent our sponsorship packages out to companies who expressed interest in our project.
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Descriptive Title of What You're Doing
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