From 2009.igem.org
(Difference between revisions)
|
|
Line 447: |
Line 447: |
| <br> | | <br> |
| <div class="heading"> | | <div class="heading"> |
- | Descriptive Title of What You're Doing
| + | Working on Canada Day |
| </div> | | </div> |
| <br> | | <br> |
| <div class="desc"> | | <div class="desc"> |
| </html> | | </html> |
- | WIKI CODING HERE
| + | I replied back to company emails and tried my best to answer their questions about our project. I researched a few old companies who had declined our proposals and searched new contacts in different departs. I will call them tomorrow. |
| | | |
| <html> | | <html> |
Revision as of 07:36, 21 August 2009
University of Calgary
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
CHINMOYEE
Descriptive Title of What You're Doing
|
|
EMILY
Descriptive Title of What You're Doing
|
|
FAHD
Descriptive Title of What You're Doing
|
|
IMAN
Descriptive Title of What You're Doing
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
KATIE
Descriptive Title of What You're Doing
|
|
KEVIN
Other hyperactive antifreeze proteins
- High Arctic plant rhizosphere(P. putida)
- Mid-gut of frozen beetle larvae (R. erythropolis)
- Frozen/chilled pork sausages (M. cryophilus)
- Antarctic soil (Moraxella sp and P. fluorescens).
None were from bacteria until MpAFP was found
|
|
MANDY
Descriptive Title of What You're Doing
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
PRIMA
Working on Canada Day
I replied back to company emails and tried my best to answer their questions about our project. I researched a few old companies who had declined our proposals and searched new contacts in different departs. I will call them tomorrow.
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
VICKI
Colony PCR of J13002 + JuxOD47A BBk + B0015 on pSB1AK3 that I prepared yesterday
Purpose: The bacterial culture growth is prolific. This will verify if the proper gene constructs are included within.
Materials an methods:
- Primers: BBk forward and reverse construction primers
- Master mix: Use pTaq DNA polymerase. The contents will suffice for 15 tubes, and were prepared in the same fashion as on June 26.
- PCR conditions: These are also the same as they were on June 26
Results:
The 1% agarose gel is attached below. Lane 1 is a GeneElute 1kb+ DNA ladder; lanes 2-11 are the colony-PCR'd samples of J13002 + LuxOD47A + B0015; lane 12 is a sequenced LuxOD47A + B0015 for a size control; lane 13 is a sequenced LuxOD47A BBk for another size control; and lane 14 is a negative control. Based on a very confusing negative control, this procedure will be repeated tomorrow. We will still progress with a NotI restriction digest of the contents, which will also be done tomorrow.
|