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Team:Calgary/14 July 2009
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| - | + | Cold Calls & Transformation of Cl lambda into xl gold | |
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| - | + | For marketing: | |
| + | The marketing team sat down and decided that we should have our third bake on Monday, July 27th and the fourth one on Monday, August 16th. These dates are tentative. Fahd and I have worked on the July newsletter template: cedarlane is our sponsor of the month. Our goal is to get it done by Friday so we can start sending it out next week. | ||
| + | For companies: | ||
| + | Company 1 – got an email back saying they’re considering our sponsorship packgage follow up with them on Monday, July 20th | ||
| + | Company 2– couldn’t get a hold of them call back tomorrow | ||
| + | Company 3 – considering our package, needs some time follow up on Monday July 20th | ||
| + | Company 4 – sent package yesterday, will follow up tomorrow to see if they got my email | ||
| + | Company 5 – (this was one of the older companies who said no…but I got a hold of someone new) –sent sponsorship package yesterday – will follow up tomorrow to see if they received it | ||
| + | Company 6 –package sent to the executive committee, now considering follow up July 20th | ||
| + | Company 7 – sent package follow up tomorrow to see if they received it | ||
| + | Company 8- got an email today …refused to help us. I Sent them a thank you letter anyways and asked for contacts | ||
| + | Company 9 - sent pkg, have CEO’s number, called before couldn’t get a hold of him, will call again tomorrow – I wanna set up a meeting with him cuz I think I know where their main office is located. | ||
| + | Tomorrow – call the 4 companies from Mrs. Kubik’s company contacts. They were Suncore, Syncrude and 2 more whose names I don’t remember and Fahd took the list home. I sent them our package 2 weeks ago, tomorrow I’ll follow up to see if they had considered sponsoring us. | ||
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| + | Lab: | ||
| + | I helped Jeremy set up sequencing for LuxPQ-B-R-LuxOU-B in AK for colony 12. We used colony 12 because the restriction digest from yesterday worked perfectly for that colony (correct size and no random fragments) (please refer to attachment). Since last time we only got one colony from transforming Cl lambda couple days ago and the restriction digest we ran yesterday showed multiple bands, we couldn’t judge the size of Cl lambda properly. Therefore, today we decided to transform Cl lambda again into competent cells. Since the inverter plasmid is resistant to Kan, we made more Kan agar plates and then transformed the Cl lambda and let them grow over night. We also made overnight cultures of the Cl lamda. We’ll isolate the plasmids tomorrow. Then in the afternoon, if we see single colonies on our overnight plates, we ‘ll do restriction digest again to confirm that the Cl lambda is actually there in our plasmids. | ||
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Revision as of 05:55, 21 August 2009
UNIVERSITY OF CALGARY
