From 2009.igem.org
(Difference between revisions)
|
|
| Line 443: |
Line 443: |
| | <br> | | <br> |
| | <div class="heading"> | | <div class="heading"> |
| - | Lots of things completed
| + | Continuation of Marketing |
| | </div> | | </div> |
| | <br> | | <br> |
| | <div class="desc"> | | <div class="desc"> |
| | </html> | | </html> |
| - | Quite a few of the stations proposed last week were completed, need to brainstorm some other ones and fix bugs as well.
| + | I shadowed Carol while she did restriction digest. She was doing construction. Her vector was apsB1AK3. However, since I didn't go into the lab very often, I wasn't sure what she was digesting. I just watched the processes and she just explained the restriction sites. |
| | | | |
| | <html> | | <html> |
Revision as of 09:10, 21 August 2009
University of Calgary
|
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
|
CHINMOYEE
Descriptive Title of What You're Doing
|
|
|
EMILY
Descriptive Title of What You're Doing
|
|
|
FAHD
Descriptive Title of What You're Doing
|
|
|
IMAN
Descriptive Title of What You're Doing
|
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
|
KATIE
Descriptive Title of What You're Doing
|
|
|
KEVIN
Descriptive of What You're Doing
|
|
|
MANDY
Descriptive Title of What You're Doing
|
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
|
PRIMA
Continuation of Marketing
I shadowed Carol while she did restriction digest. She was doing construction. Her vector was apsB1AK3. However, since I didn't go into the lab very often, I wasn't sure what she was digesting. I just watched the processes and she just explained the restriction sites.
|
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
|
VICKI
Colony PCR of J13002+LuxOD47A and LuxOD47A+B0015 bacteria
Purpose: Cultures grew successfully on plates last night. This will help confirm which of those colonies took up the plasmids that we wanted to, and that the plasmids that were taken up include properly-constructed genes in the right order.
Protocol: The colony PCR was conducted in accordance with that outlined on the protocol page. Platinum Taq polymerase was used, along with BBk construction primers. 5 colonies were tested for each construct, along with one negative control (for a total of 11 tubes being amplified).
Results: The gel is included below. The negative control results are peculiar, but are not raising any concern as the contamination band is not found anywhere else on the gel. Some of the LuxOD47A stock was run to serve as a size comparison. From this, it appears that the LuxOD47A+B0015 parts were successfully constructed and transformed, along with colonies 2 and 4 of the J13002+LuxOD47A parts.
|
"
