Team:Aberdeen Scotland/notebook/quorumsensing

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(Week 1 - Research and Biobrick Rescues)
(Week 1 - Research and Biobrick Rescues (08/06/09 - 12/06/09))
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==Week 1 - Research and Biobrick Rescues (08/06/09 - 12/06/09)==
==Week 1 - Research and Biobrick Rescues (08/06/09 - 12/06/09)==
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<br><br>
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===Day 1 Monday (08/06/09)===
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*Researching the Registry for Biobricks
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**Focus on QS module
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**Identified K081008, J23100, C0062, K091156, B0034,B0030, B0015, R0061 and R0062 for possible use in our project.
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**Other biobricks identified for other sub-projects include R0011.
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<br><br>
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===Day 2 Tuesday (09/06/09)===
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*Researching the literature for information on Quorum Sensing.
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*Researching for information on LVA tags
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<br><br>
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===Day 3 Wednesday (10/06/09)
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*Preparing for biobrick rescue and transformation
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**Prepare LB and Agar
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**Prepare solutions
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***Cacl2 solution,
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***1M MgCl2, 1MMgSO4 and 2M glucose solutions (for SOC medium)
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**Inoculate e.coli and grow overnight
==Week 2 15/06/09 - 19/06/09==
==Week 2 15/06/09 - 19/06/09==

Revision as of 13:12, 21 August 2009

University of Aberdeen iGEM 2009


Contents

Quorum Sensing Notebook

Week 1 - Research and Biobrick Rescues (08/06/09 - 12/06/09)



Day 1 Monday (08/06/09)

  • Researching the Registry for Biobricks
    • Focus on QS module
    • Identified K081008, J23100, C0062, K091156, B0034,B0030, B0015, R0061 and R0062 for possible use in our project.
    • Other biobricks identified for other sub-projects include R0011.



Day 2 Tuesday (09/06/09)

  • Researching the literature for information on Quorum Sensing.
  • Researching for information on LVA tags



===Day 3 Wednesday (10/06/09)

  • Preparing for biobrick rescue and transformation
    • Prepare LB and Agar
    • Prepare solutions
      • Cacl2 solution,
      • 1M MgCl2, 1MMgSO4 and 2M glucose solutions (for SOC medium)
    • Inoculate e.coli and grow overnight

Week 2 15/06/09 - 19/06/09

Week 3 22/06/09 - 26/06/09

Week 4 29/06/09 - 03/07/09

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09