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Team:Calgary/8 July 2009
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| - | + | Today was a day of plasmid isolation and glycerol stock preparation. I made overnight cultures and restreaks last night of the parts that I had biobricked. We need some isolated DNA for the registry, as well as glycerol stocks for long-term storage. We have samples of everything that I prepared, with concentrations on the order of 100 ng/uL. One of the sets - the LuxO D47A BBk - was not isolated well, so I made new overnights and will prepare glycerol stocks of those tomorrow. | |
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Revision as of 19:57, 21 August 2009
UNIVERSITY OF CALGARY
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Adjusting Model frame work
Me , Carol and Kevin adjusted our model frame work . Then the whole modelling team had a good discussion with Thane . Before that I read more journals dealing with parameter estimation and looked for rates . I looked at the parameter fit function in simbiology and tried to use this function for our purpose.
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EMILY
DNA Sequencing and cloning of B0015
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FAHD
Descriptive Title of What You're Doing
WIKI CODING HERE
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IMAN
Descriptive Title of What You're Doing
WIKI CODING HERE
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JAMIE
Descriptive Title of What You're Doing
WIKI CODING HERE
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JEREMY
Descriptive Title of What You're Doing
WIKI CODING HERE
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KATIE
Creation and Modification of Scripts and Notecards
I have completed creating instructional and information note cards for the various activities within the virtual lab on our island, including:
Note cards that are almost finished and will be completed by tomorrow include:
I have also been creating and modifying the scripts for PCR, Gel Electrophoresis, Restriction Digest and have just started Bacterial Transformation. They have been modified to use a more user friendly prompt system (using llDialog function in second life scripting language) so asking avatars for information and having them answer looks cleaner and is more intuitive.
I am hoping to have restriction digest functioning by Friday and will be working on finishing notecards, Restriction Digest and Bacterial Transformation in the upcoming days. I will also be organizing a sequencing station, which may be used for a section of the lab activities.
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KEVIN
Restreak of the glowing cells
A brightest colony from yesterday's transformation were restreaked. These cells have to grow overnight, once again.
Blog update
A weekly blog is being done by all sections of our team, including wetlab and modelling which I am part of. Every wednesday is wetlab blog day, so one was written. The following is the link to my post:
Kevin Loves Rainbows
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MANDY
Descriptive Title of What You're Doing
WIKI CODING HERE
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PATRICK
Descriptive Title of What You're Doing
WIKI CODING HERE
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PRIMA
Marketing
I began looking at Westjet and Air Canada flights flying to Boston. I had callled a few travel agencies to try to get a quote. I also looked at Hotels at MIT. I need to detmerine how far it is from MIT, whether it would be economical to stay there and take a cab or get a hotel closer (but a little bit more expensive) and walk to MIT. I also made a list of hotels including their rates, discounts, services, distance from MIT, etc.
I began following up with comapnies via phone and email. I found 16 mor companies that I could contact. I began researching those companies and writing up emails to them. The team also sat down to discuss logo designs, colors, shapes, etc.
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STEFAN
Descriptive Title of What You're Doing
WIKI CODING HERE
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VICKI
Glycerol stock preparation of successfully-biobricked parts
Today was a day of plasmid isolation and glycerol stock preparation. I made overnight cultures and restreaks last night of the parts that I had biobricked. We need some isolated DNA for the registry, as well as glycerol stocks for long-term storage. We have samples of everything that I prepared, with concentrations on the order of 100 ng/uL. One of the sets - the LuxO D47A BBk - was not isolated well, so I made new overnights and will prepare glycerol stocks of those tomorrow.
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