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Team:Calgary/21 May 2009
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| - | + | *Today we visualized our PCR from yesterday. We learned how to prepare agarose gels, load gels and visualize them in the G-Box. See photo of gel below. Lanes 1-4 is Kevin's Pqrr4, lane 5 is Kevin's negative control, lanes 6-7 are left blank, lane 8 is a negative control and lanes 9-12 are LuxOD47E colony 2. | |
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| + | *Analysis: It is good that the negative control is clean as this indicates that there is no contamination. The presence of bands also indicates that we were able to apmplify something, also good! It looks like LuxOD47E is likely there, as we see band around the expected size of 1.4 kb, however the lanes appear to be overloaded with DNA, so it is not conclusive. The bands are not very clean, so we will try this PCR gain next week. | ||
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Revision as of 02:15, 13 September 2009
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CAROL
Verification of amplification of luxCDABE from polymerase chain reaction (PCR)
RESULTS:
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CHINMOYEE
CLASS
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EMILY
Visualization of PCR Product
File:2009.05.21Pqrr4-LuxOD47E.jpeg.jpeg
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FAHD
WHIMS TRAINING and First Sponsorship May 21st 2009
We had our WHIMS training today at the University of Calgary Main Campus. It is a required course to take if we are working in a lab. In the afternoon, I was greeted with some great news: NEB (New England BioLabs) Canada had agreed to sponsor us with lab equipment and reagents. It was our first ever sponsorship. It was also an exciting moment for our entire team as it would save us loads and loads of money because our WetLab team uses a lot of reagents from NEB Canada.
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IMAN
Descriptive Title of What You're Doing
WIKI CODING HERE
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JAMIE
Descriptive Title of What You're Doing
WIKI CODING HERE
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JEREMY
Descriptive Title of What You're Doing
WIKI CODING HERE
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KATIE
Planning PCR Activity
Started to make an outline of what I will be required to do for PCR machine:
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KEVIN
WHMIS traning
The whole morning was spent learning about safety.
Logo
In the afternoon, we brainstormed about our team logo.
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MANDY
Descriptive Title of What You're Doing
WIKI CODING HERE
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PATRICK
Early Development
Retrospective Notebook: This entry was not written on this day, but derived later from working notes I made that day.
First came up with the polymer linking system that I would eventually use without too much modification: each piece of DNA knows the name of the next piece in the polymer. Figuring out what to use as that 'name', and how to store it, would be a much longer process. I noticed very quickly that the internal identifier assigned by SL to each object changed every time the object was copied from the user's inventory, so would not be suitable for tracking polymer structure over time. Began to notice how important a consistent scheme for inter-object communication would be. Ultimately, the objects that make up the Biobrick simulator would send something like 70 distinct message types, a number which is only set to increase. In the case of the HUD, there would be more than a hundred possible destination objects! Decided that as a first working goal, I would try to implement the TetR operon, something that as of this writing (August 20 2009) I have not yet fully completed!
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PRIMA
Marketing
We had the WHMIS training on main campus all morning (9:00am - 12:00 noon).
In the afternoon, I continued my follow-ups with companies via email. Jeremy, Vicki and I wrote up Thank You letters to the Dragons from Dragon's Den for taking the time out of their busy schedules to watch our marketing pitches, provide feedback, advising us on marketing and business initiatives and way to sell our project. I researched a few more companies that I had found, wrote up emails and updated the Gmail marketing document.
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STEFAN
Descriptive Title of What You're Doing
WIKI CODING HERE
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VICKI
Descriptive Title of What You're Doing
WIKI CODING HERE
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