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Template:Team:KULeuven/11 August 2009/VanillinReceptor
From 2009.igem.org
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| - | * We also | + | * We also received another strain of ''Agrobacterium''. We brought this new strain in culture. |
| - | * | + | * To make things clear the genes from the first ''Agrobacterium'' will from now on be called A,B,G and R for respectively virA, promotor region virB , virG and rpoA. For the second ''Agrobacterium'' we will use W,X,Y and Z to indicate the same genes. |
| - | * We achieved good results for genes B, G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with | + | * We achieved good results for genes B,G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampicillin. We isolated the good colonies again. |
* There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A. | * There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A. | ||
Revision as of 08:46, 8 September 2009
- We also received another strain of Agrobacterium. We brought this new strain in culture.
- To make things clear the genes from the first Agrobacterium will from now on be called A,B,G and R for respectively virA, promotor region virB , virG and rpoA. For the second Agrobacterium we will use W,X,Y and Z to indicate the same genes.
- We achieved good results for genes B,G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampicillin. We isolated the good colonies again.
- There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A.
