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Template:Team:KULeuven/25 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | * miniprepping the liquid cultures with pSB3K3 | |
{| border ="1" align="center" | {| border ="1" align="center" | ||
!| Part || concentration (ng/μl) || 260/280 λ || | !| Part || concentration (ng/μl) || 260/280 λ || | ||
| Line 7: | Line 7: | ||
| pSB3K3 (2) || 47,7 || 2,08 || | | pSB3K3 (2) || 47,7 || 2,08 || | ||
|} | |} | ||
| - | + | *restriction digest of pSB3K3 with EcoRI and PstI | |
| - | + | *gel electroforesis and extraction of the RD of pSB3K3 | |
--> failed. there was no signal at all | --> failed. there was no signal at all | ||
| - | + | *new colonies were ented in liquid culture. | |
| - | + | *ligation C performed: J23101 (A) + E0240 | |
| + | {| border ="1" align="center" | ||
| + | |- align="center" | ||
| + | | Vector || insert | ||
| + | |- align="center" | ||
| + | | {{kulpart|psB1A3}}+ E0240 || {{kulpart|bba_J23101}} || | ||
| + | |- align="center" | ||
| + | | 30ng -> 1,4μl || 40,8ng -> 2μl | ||
| + | |- align="center" | ||
| + | |} | ||
Revision as of 13:50, 25 August 2009
- miniprepping the liquid cultures with pSB3K3
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| pSB3K3 (1) | 27,6 | 1,91 | |
| pSB3K3 (2) | 47,7 | 2,08 |
- restriction digest of pSB3K3 with EcoRI and PstI
- gel electroforesis and extraction of the RD of pSB3K3
--> failed. there was no signal at all
- new colonies were ented in liquid culture.
- ligation C performed: J23101 (A) + E0240
| Vector | insert | |
| + E0240 | ||
| 30ng -> 1,4μl | 40,8ng -> 2μl |
