Template:Team:KULeuven/18 August 2009/VanillinReceptor

From 2009.igem.org

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* PCR purification miniprep and nanodrop of R on mutation 2
* PCR purification miniprep and nanodrop of R on mutation 2
-
* start ethanol precipitatie (overnight ethanol + sodium acetate)to create a better result(more purified product) of product R with mutation 202
+
* Start ethanol precipitation (overnight ethanol + sodium acetate) to create a better result (more purified product) of product R with mutation 202
-
* PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelelectroforesis showed nothing so TOPO cloning failed again for virA.
+
* PCR of the fragments of TOPO (W and Z) was done to check if TOPO worked. Gelelectrophoresis showed nothing so TOPO cloning failed again for ''virA''.

Latest revision as of 09:24, 14 September 2009

  • PCR purification miniprep and nanodrop of R on mutation 2
  • Start ethanol precipitation (overnight ethanol + sodium acetate) to create a better result (more purified product) of product R with mutation 202
  • PCR of the fragments of TOPO (W and Z) was done to check if TOPO worked. Gelelectrophoresis showed nothing so TOPO cloning failed again for virA.