Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→Blue light receptor)
(→Blue light receptor)
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| MC4100 || / || strain of E.coli || || || || july 24
| MC4100 || / || strain of E.coli || || || || july 24
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| 2171 || / ||forward primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM ||july 24
 
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| 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM || july 24
 
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| 2171 || / || forward primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24
 
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| 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24
 
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| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || 42,5µl || 20μM ||aug 10
| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || 42,5µl || 20μM ||aug 10
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| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM || aug 10
| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM || aug 10
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|-
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| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || || 100μM || aug 10
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| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||Miniprepped||100μl||85,6ng/μl||july 29
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| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || || 100μM || aug 10
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| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||restricted with EcoRI and XbaI|| 20μl ||||july 29
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| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||restricted with EcoRI and XbaI||||||july 29
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| {{kulpart|BBa_K145015}}||pSB1A2||GFP + LVA-tag + terminator||restricted with EcoRI and XbaI||30μl||29,3 ng/μl|| aug 14
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| {{kulpart|BBa_K145015}}||pSB1A2||GFP + LVA-tag + terminator||restricted with EcoRI and XbaI||||||july 29
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| {{kulpart|BBa_K238000}}||/||BLR promoter region (1:200 adn 1:500)||restricted with EcoRI||||||july 29
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| {{kulpart|BBa_K238000}}||/||PCR BLR promoter region (360bp)||4 microcentrifuge tubes||||||july 30
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| {{kulpart|BBa_pSB3K3-P1010}}|| pSB3K3 ||kanamycine plasmide backbone|| taken from plates || 13μl ||||aug 11
| {{kulpart|BBa_pSB3K3-P1010}}|| pSB3K3 ||kanamycine plasmide backbone|| taken from plates || 13μl ||||aug 11
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| {{kulpart|BBa_K238013}}||/||Exact blue light promotor ||pcr product|| / || 123,3 ng/μl ||aug 13
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| {{kulpart|BBa_K238013}}||/||Exact blue light promotor ||pcr product|| 20μl || 123,3 ng/μl ||aug 13
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| {{kulpart|BBa_J23101}}||/||constitutive promotor||taken from plates || 13μl ||||aug 11
| {{kulpart|BBa_J23101}}||/||constitutive promotor||taken from plates || 13μl ||||aug 11
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| {{kulpart|BBa_K238013+ BBa_K145015}}||/||Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 ||T4 DNA ligation || 18μl || ||aug 14
| {{kulpart|BBa_K238013+ BBa_K145015}}||/||Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 ||T4 DNA ligation || 18μl || ||aug 14
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| {{kulpart|BBa_K238013+ BBa_E02040}}||/||Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9) ||cut with EcoRI and PstI || 5 x 18μl || ||aug 18
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| {{kulpart|BBa_K238013+ BBa_E02040}}||/||Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,7,9) ||cut with EcoRI and PstI || 4 x 18μl || ||aug 24
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| {{kulpart|BBa_K238013+ BBa_E0240}}||/||LigA: Exact blue light promotor ligated with E0240 in pSB1A2 ||Miniprep (4x)|| 20μl|| ||aug 18
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| {{kulpart|BBa_J23101}}||pSB1A3||constitutive promotor||miniprep||10μl||141,2ng/μl||aug 14
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| {{kulpart|BBa_J23101}}(A)||||constitutive promotor||restriction digest with EcoRI en SpeI|| 20μl ||20,8ng/μl||aug 24
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| {{kulpart|BBa_J23101}}(B)||||constitutive promotor||restriction digest with EcoRI en SpeI|| 10μl ||12,8ng/μl||aug 24
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Revision as of 13:47, 29 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2260 / forward primer for 42,5µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
pSB1A2GFP + rbs + terminatorMiniprepped100μl85,6ng/μljuly 29
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaI 20μl july 29
pSB1A2GFP + LVA-tag + terminatorrestricted with EcoRI and XbaI30μl29,3 ng/μl aug 14
pSB3K3 kanamycine plasmide backbone taken from plates 13μl aug 11
/Exact blue light promotor pcr product 20μl 123,3 ng/μl aug 13
/constitutive promotortaken from plates 13μl aug 11
/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl aug 12
/LigA: Exact blue light promotor ligated with E0240 in pSB1A2 T4 DNA ligation 18μl aug 13
/Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 T4 DNA ligation 18μl aug 14
/Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,7,9) cut with EcoRI and PstI 4 x 18μl aug 24
/LigA: Exact blue light promotor ligated with E0240 in pSB1A2 Miniprep (4x) 20μl aug 18
pSB1A3constitutive promotorminiprep10μl141,2ng/μlaug 14
(A)constitutive promotorrestriction digest with EcoRI en SpeI 20μl 20,8ng/μlaug 24
(B)constitutive promotorrestriction digest with EcoRI en SpeI 10μl 12,8ng/μlaug 24

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (oranje)108,8 ng/µlAugust 4
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (oranje)101,3 ng/µlAugust 5
pSB1A2COMTpurified plasmid (oranje)122,9 ng/µlAugust 5
pSB1A2fcs + rbspurified plasmid (fcs pl.)29 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)31 µl76,0 ng/µlAugust 12
pSB1A2ech + rbspurified plasmid (ech B)42,8 µl96,7 ng/µlAugust 19
pSB1A2ech + rbspurified plasmid (ech C)48 µl105,6 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs B)43,6 µl113,4 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs C)48 µl90,3 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 B)44 µl125,0 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 C)48 µl104,2 ng/µlAugust 19
pSB1A2Sam8 + rbspurified plasmid (sam8 B)40,1 µl63,2 ng/µlAugust 19
pSB1A2sam8 + sam5ligation mix (SAMS)August 24
pSB1A2fcs + echligation mix (EF)August 24
pSB1A2fcs + echpurified ligation plasmid (EF I)48 µl53,9 ng/µlAugust 4
pSB1A2fcs + echpurified ligation plasmid (EF II)42 µl39,4 ng/µlAugust 4
pSB1A2fcs + echpurified ligation plasmid (EF III)39,9 µl57,1 ng/µlAugust 4
pSB1A2sam8 + sam5purified ligation plasmid (SAMS I)48 µl76,8 ng/µlAugust 4
pSB1A2sam8 + sam5purified ligation plasmid (SAMS II)35,383,4 ng/µlAugust 4
pSB1A2sam8 + sam5purified ligation plasmid (SAMS III)39,3 µl61,5 ng/µlAugust 4

vanillin receptor

part plasmid description status μl left concentration date
2235 / forward primer for 20μM August 3
2238 / reverse primer for 20μM August 3
2235 / forward primer for 100μM August 3
2238 / reverse primer for 100μM August 3
2242 / forward primer for 20μM August 3
2243 / reverse primer for 20μM August 3
2242 / forward primer for 100μM August 3
2243 / reverse primer for 100μM August 3
2244 / forward primer for 20μM August 3
2245 / reverse primer for 20μM August 3
2244 / forward primer for 100μM August 3
2245 / reverse primer for 100μM August 3
2246 / forward primer for 20μM August 3
2247 / reverse primer for 20μM August 3
2246 / forward primer for 100μM August 3
2247 / reverse primer for 100μM August 3
2288 / forward primer for mutagenesis for 20μM August 8
2289 / reverse primer for mutagenesis for 20μM August 8
2288 / forward primer for mutagenesis for 100μM August 8
2289 / reverse primer for mutagenesis for 100μM August 8
2290 / forward primer for mutagenesis for 20μM August 8
2291 / reverse primer for mutagenesis for 20μM August 8
2290 / forward primer for mutagenesis for 100μM August 8
2291 / reverse primer for mutagenesis for 100μM August 8
2292 / forward primer for mutagenesis for 20μM August 8
2293 / reverse primer for mutagenesis for 20μM August 8
2292 / forward primer for mutagenesis for 100μM August 8
2293 / reverse primer for mutagenesis for 100μM August 8
2294 / forward primer for mutagenesis for 20μM August 8
2295 / reverse primer for mutagenesis for 20μM August 8
2294 / forward primer for mutagenesis for 100μM August 8
2295 / reverse primer for mutagenesis for 100μM August 8

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline
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