Team:TUDelft/27 August 2009

From 2009.igem.org

(Difference between revisions)
(→Saeed)
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Line 16: Line 16:
| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||  
| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||  
|- align="center"
|- align="center"
-
| 2 || !Fr1n upstream || 1900 || ok, but not needed
+
| 2 || !a || 1934 || no band
|- align="center"
|- align="center"
-
| 3 || !Fr1n downstream || 1900 || ok
+
| 3 || !Zb || 1934 || ok
|- align="center"
|- align="center"
-
| 4 || destination plasmid || 3000 || ok
+
| 4 || !Zg || 1934 || ok
 +
|- align="center"
 +
| 5 || !Zh || 1934 || ok
 +
|- align="center"
 +
| 6 || DNA ladder ||  ||
|}
|}

Revision as of 14:17, 28 August 2009


27 August 2009

Saeed

The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.

Gelsaeed27-082009 !Z.wiki.jpg

lane Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
2  !a 1934 no band
3  !Zb 1934 ok
4  !Zg 1934 ok
5  !Zh 1934 ok
6 DNA ladder




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