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Team:Aberdeen Scotland/Registry
From 2009.igem.org
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'''[http://partsregistry.org/Part:BBa_I13600 BBa_I13600]''' | '''[http://partsregistry.org/Part:BBa_I13600 BBa_I13600]''' | ||
| + | <!-- Aberdeen_Scotland 2009 user review --> | ||
| + | {|width='80%' style='border:1px solid gray' | ||
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| + | <partinfo>BBa_I13600 AddReview 5</partinfo> | ||
| + | <I> Aberdeen_Scotland 2009 </I> | ||
| + | |width='60%' valign='top'| | ||
| + | <html><p align="justify"> | ||
| + | The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. | ||
| + | |} | ||
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'''[http://partsregistry.org/Part:BBa_I732820 BBa_I732820]''' | '''[http://partsregistry.org/Part:BBa_I732820 BBa_I732820]''' | ||
Revision as of 14:03, 30 August 2009
University of Aberdeen - Pico Plumber
BioBrick Experience
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]
|
Aberdeen_Scotland 2009 |
The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing.The plasmid miniprep also worked. |
[http://partsregistry.org/Part:BBa_C0040 BBa_C0040]
|
Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the EcoRI and SpeI) worked as expected. |
[http://partsregistry.org/Part:BBa_S03518 BBa_S03518]
|
Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the EcoRI and SpeI) worked. Further testings were performed by the formation of the BBa_K182002, PCR analysis, sequencing and everything worked flawlessly according to our expectation. |
[http://partsregistry.org/Part:BBa_I732100 BBa_I732100]
|
Aberdeen_Scotland 2009 |
The plasmid miniprep and the gel worked as expected. However, since we did not use this part for our cloning step we cannot comment on the sequencing or the other features of this part. |
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
|
Aberdeen_Scotland 2009 |
The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (54bp).But later it was confirmed following its usage by forming the part BBa_K182001 through the PCR gel analysis and the sequencing.The plasmid miniprep also worked. |
[http://partsregistry.org/Part:BBa_I13600 BBa_I13600]
|
Aberdeen_Scotland 2009 |
The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. |} '''[http://partsregistry.org/Part:BBa_I732820 BBa_I732820]''' '''[http://partsregistry.org/Part:BBa_I13601 BBa_I13601]''' '''[http://partsregistry.org/Part:BBa_K081008 BBa_K081008]''' '''[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]''' '''[http://partsregistry.org/Part:BBa_E0840 BBa_E0840]''' '''[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]''' '''[http://partsregistry.org/Part:pSB1AT3 pSB1AT3]'''
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