Team:Chiba/Notebook/Calendar/2 September 2009

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Revision as of 13:02, 3 September 2009

プレ培養(予備実験)/prior culture(exploratory experiment)

使った培地：Amp耐性、液体/medium:liquid culture medium antibiotic:Amp

この溶液50 mLを遠心管にとり、更に抗生物質(Cm)を50 μLを加え、Amp-Cm液体培地とした。/We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 uL.

これを試験管に2 mLとり、グリセロールストック(BW25113株、Δtdk、pcIA3-LuxR、pLux-GFP)を添加し37℃で振とうした。(21:40-)/Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) then started shake culture at 37 degrees Celsius.

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	この溶液50 mLを遠心管にとり、更に抗生物質(Cm)を50 μLを加え、Amp-Cm液体培地とした。/We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 uL.		この溶液50 mLを遠心管にとり、更に抗生物質(Cm)を50 μLを加え、Amp-Cm液体培地とした。/We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 uL.

-	これを試験管に2 mLとり、グリセロールストック(BW25113株、Δtdk、pcIA3-LuxR、pLux-GFP)を添加し37℃で振とうした。(21:40-)/Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ~~&deltaTdk~~,pCIA3-LuxR,pLux-GFP) then started shake culture at 37 degrees Celsius.	+	これを試験管に2 mLとり、グリセロールストック(BW25113株、Δtdk、pcIA3-LuxR、pLux-GFP)を添加し37℃で振とうした。(21:40-)/Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) then started shake culture at 37 degrees Celsius.