"
Team:KULeuven/21 August 2009
From 2009.igem.org
(Difference between revisions)
Bart Bosmans (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/BeginHeader}} |
{{Team:KULeuven/Common/SubMenu_Notebook}} | {{Team:KULeuven/Common/SubMenu_Notebook}} | ||
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/EndHeader}} |
{{Team:KULeuven/Notebook/DayNavigator}} | {{Team:KULeuven/Notebook/DayNavigator}} | ||
| Line 14: | Line 14: | ||
{{Team:KULeuven/EditableTemplateH3|Vanillin Receptor|{{PAGENAME}}/VanillinReceptor}} | {{Team:KULeuven/EditableTemplateH3|Vanillin Receptor|{{PAGENAME}}/VanillinReceptor}} | ||
{{Team:KULeuven/EditableTemplateH3|Key/Lock/Anti-Key|{{PAGENAME}}/KeyLockAntiKey}} | {{Team:KULeuven/EditableTemplateH3|Key/Lock/Anti-Key|{{PAGENAME}}/KeyLockAntiKey}} | ||
| + | {{Team:KULeuven/Common2/PageFooter}} | ||
Latest revision as of 08:48, 10 September 2009
Project progress
Progress of parts
[edit] Blue Light Receptor
[edit] Vanillin Production
- Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ech-plasmid. The signals do not correspond with an EF-ligation product.
- The DNA was purified from the gel
Nanodrop results:
| Part | ng/µl | 260/280 | |
|---|---|---|---|
| sam8 | 2,5 | 1,14 | |
| sam5 | 7,9 | 1,60 | |
| ech | 5,0 | 1,38 | |
| fcs | 3,4 | 0,93 |
- sam8 and sam5; fcs and ech were ligated
Used volumes:
| Part | µl | |
|---|---|---|
| sam8 | 34 | |
| sam5 | 6,3 | |
| ech | 10,0 | |
| fcs | 35,8 |
[edit] Vanillin Receptor
- TOPO cloning of W looks goods with clear white colonies. 5 colonies were selected and cultured for testing
