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Team:Imperial College London/Wetlab/Protocols
From 2009.igem.org
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*The cells will be grown until OD= 0.7. | *The cells will be grown until OD= 0.7. | ||
| - | *The RFP value will be monitered, although it is the GFP values that are more critical in this assay. | + | *The RFP value will be monitered, although it is the GFP values that are more critical in this assay. |
| - | *OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs) | + | *OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)]. |
| - | + | ||
| - | *The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter) | + | *The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter) |
| - | *The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG) | + | *The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG) |
| - | [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|See Glucose time delay for details]] | + | [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|See Glucose time delay for details]] |
=Protein production assays= | =Protein production assays= | ||
Revision as of 13:49, 10 September 2009
Contents |
Autoinduction assays
Glucose time delay
Aims
- Characterise the tunable time duration it takes before GFP expression (M2 activation)
Assay
- The cells will be grown until OD= 0.7.
- The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
- OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
- The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
- The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
See Glucose time delay for details
Protein production assays
Encapsulation assays
Genomic deletion assays
